PDF(Pubmed)
Abstract：
SARS-CoV-2 is the virus responsible for the COVID-19 pandemic, causing respiratory syndrome and other manifestations. The clinical consequences of the SARS-CoV-2 infection are highly heterogeneous, ranging from asymptomatic and mild to severe and fatal conditions, with the highest mortality rate reached among elderly people. Such heterogeneity appears strongly influenced by the host immune response, which in turn is profoundly affected by aging. In fact, the occurrence of a low-grade inflammation and a decline in specific immune defense is generally reported in older people. Although the low ability of B cells to provide primary and secondary specific responses with a consequent increase in susceptibility to and severity of virus infections is generally described in elderly people, we would like to present here the particular case of a 100-year-old woman, who recovered well from COVID-19 and developed a long-term memory against SARS-CoV-2. Following the infection, the patient\'s blood was tested with both a classical ELISA and a specific Cell-ELISA addressed to measure the anti-spike S1 specific IgG released in plasma or produced in vitro by memory B cells, respectively. While showing negative on classical serological testing, the patient\'s blood was positive in Cell-ELISA up to 1 year after the infection. Our observation highlights a potential mechanism of B cell-dependent, long-term protection in response to SARS-CoV-2 infection, suggesting that in a case of successful aging, the absence of specific antibodies in serum does not necessarily mean the absence of immune memory.
摘要：
SARS-CoV-2是导致COVID-19大流行的病毒，引起呼吸综合征和其他表现。SARS-CoV-2感染的临床后果是高度异质性的，从无症状和轻度到严重和致命的情况，老年人死亡率最高。这种异质性似乎受到宿主免疫反应的强烈影响，这反过来又受到衰老的深刻影响。事实上,低度炎症的发生和特异性免疫防御的下降通常在老年人中报道.尽管在老年人中通常描述了B细胞提供初级和次级特异性反应的能力低，从而增加了对病毒感染的易感性和严重程度，我们想在这里介绍一个100岁女性的特殊案例，他从COVID-19中恢复得很好，并对SARS-CoV-2产生了长期记忆。感染后,用经典ELISA和特异性细胞ELISA对患者的血液进行了测试，以测量血浆中释放的或由记忆B细胞体外产生的抗尖峰S1特异性IgG，分别。虽然在经典血清学测试中显示阴性，感染后1年，患者的血液在细胞ELISA中呈阳性。我们的观察强调了B细胞依赖性的潜在机制，应对SARS-CoV-2感染的长期保护，这表明在成功衰老的情况下，血清中缺乏特异性抗体并不一定意味着缺乏免疫记忆。
