PDF(Pubmed)
Abstract：
Silver nanoparticles (AgNPs) have stable reactivity and excellent optical absorption properties. They can be applied in various industries, such as environmental protection, biochemical engineering, and analyte monitoring. However, synthesizing AgNPs and determining their appropriate dosage as a coloring substance are difficult tasks. In this study, to optimize the process of AgNP synthesis and obtain a simple detection method for trace mercury in the environment, we evaluate several factors-including the reagent addition sequence, reaction temperature, reaction time, the pH of the solution, and reagent concentration-considering the color intensity and purity of AgNPs as the reaction optimization criteria. The optimal process for AgNP synthesis is as follows: Mix 10 mM of silver nitrate with trisodium citrate in a hot water bath for 10 min; then, add 10 mM of sodium borohydride to produce the AgNPs and keep stirring for 2 h; finally, adjust the pH to 12 to obtain the most stable products. For AgNP-based mercury detection, the calibration curve of mercury over the concentration range of 0.1-2 ppb exhibits good linearity (R2 > 0.99). This study provides a stable and excellent AgNP synthesis technique that can improve various applications involving AgNP-mediated reactions and has the potential to be developed as an alternative to using expensive detection equipment and to be applied for the detection of mercury in food.
摘要：
银纳米粒子(AgNPs)具有稳定的反应性和优异的光吸收性能。它们可以应用于各种行业，如环境保护,生化工程,和分析物监测。然而,合成AgNPs和确定它们作为着色物质的适当剂量是困难的任务。在这项研究中,优化AgNP的合成工艺,获得一种简单的环境中痕量汞的检测方法,我们评估了几个因素，包括试剂添加顺序，反应温度,反应时间,溶液的pH值，和试剂浓度-考虑AgNPs的颜色强度和纯度作为反应优化标准。合成AgNP的最佳工艺如下：将10mM硝酸银与柠檬酸三钠在热水浴中混合10分钟；然后，添加10mM硼氢化钠以产生AgNPs，并保持搅拌2小时；最后，调节pH至12以获得最稳定的产品。对于基于AgNP的汞检测，在0.1-2ppb的浓度范围内，汞的校准曲线表现出良好的线性（R2>0.99）。这项研究提供了一种稳定且出色的AgNP合成技术，可以改善涉及AgNP介导的反应的各种应用，并且有可能被开发为使用昂贵的检测设备的替代方法，并应用于食品中汞的检测。
