PDF(Pubmed)
Abstract：
The cyclical proliferation of the wild fossorial rodent Arvicola terrestris scherman (ATS) is critical in mid-mountain ecosystems of several European countries. Our goal is to develop an immunocontraceptive vaccine to control their fertility, as a sustainable alternative to chemical poisons currently used. Indeed, these chemicals cause the death of ATS predators and animals sharing their ecosystem, and current laws progressively limit their use, making the development of a targeted vaccination strategy an interesting and efficient alternative. In order to identify species-specific sperm antigens, male and female ATS received subcutaneous injections of whole ATS spermatozoa to elicit an immune response. The analysis of the immune sera led to the identification of 120 immunogenic proteins of sperm cells. Of these, 15 were strictly sperm-specific and located in different regions of the male gamete. Some of these antigens are proteins involved in molecular events essential to the reproductive process, such as sperm-egg interaction, acrosomal reaction, or sperm motility. This approach not only identified a panel of immunogenic proteins from ATS sperm cells, but also demonstrated that some of these proteins trigger an immune response in both male and female ATS. These spermatic antigens are good candidates for the development of a contraceptive vaccine.
摘要：
野生啮齿动物Arvicolaterrestrisscherman（ATS）的周期性增殖在几个欧洲国家的中山生态系统中至关重要。我们的目标是开发一种免疫避孕疫苗来控制他们的生育能力，作为目前使用的化学毒物的可持续替代品。的确,这些化学物质导致ATS捕食者和共享生态系统的动物死亡，现行法律逐渐限制其使用，使开发有针对性的疫苗接种策略成为一种有趣而有效的替代方案。为了鉴定物种特异性精子抗原，男性和女性ATS接受整个ATS精子的皮下注射以引起免疫反应。免疫血清的分析导致鉴定了120种精子细胞的免疫原性蛋白。其中,15个是严格的精子特异性的，位于雄配子的不同区域。这些抗原中的一些是参与生殖过程所必需的分子事件的蛋白质，比如精子-卵子相互作用，顶体反应,或精子活力。这种方法不仅从ATS精子细胞中鉴定出一组免疫原性蛋白，但也证明了这些蛋白质中的一些会触发男性和女性ATS的免疫反应。这些精索抗原是开发避孕疫苗的良好候选者。
