Abstract:
RNA represents a vibrant area of research and many studies use techniques that require large amounts of purified RNA. One common purification method involves slicing a section of a polyacrylamide gel containing the RNA of interest and eluting the RNA out of the gel using electroelution. Various electroeluter models are available but sometimes a given model becomes discontinued, compelling researchers to choose a different model. Here, we have compared two electroeluters with different chamber designs for their ability to recover RNA from gel pieces. Our results show that both electroeluters are effective and recover comparable amounts of purified RNA.
摘要:
RNA代表了一个充满活力的研究领域,许多研究使用需要大量纯化RNA的技术。一种常用的纯化方法包括将含有目的RNA的聚丙烯酰胺凝胶切片,并使用电洗脱将RNA从凝胶中洗脱出来。各种电子洗脱器模型是可用的,但有时一个给定的模型变得停产,迫使研究人员选择不同的模型。这里,我们已经比较了两种具有不同腔室设计的电洗脱器从凝胶块中回收RNA的能力。我们的结果表明,两种电洗脱剂都是有效的,并且可以回收相当量的纯化RNA。