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Abstract：
Introduction: Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease (IBD) and Barrett\'s esophagus (BE). Programmed cell death ligand-1 (PD-L1) is an immune checkpoint protein that is used by tumor cells for immune evasion and has increased expression in patients with IBD and BE. We examined whether C. concisus upregulates PD-L1 expression in intestinal and esophageal epithelial cells. Methods: Human intestinal epithelial HT-29 cells and esophageal epithelial FLO-1 cells with and without interferon (IFN)-γ sensitization were incubated with C. concisus strains. The level of PD-L1 mRNA was quantified using quantitative real-time PCR. Cytokines were measured using Enzyme-Linked Immunosorbent Assay (ELISA). Apoptosis of HT-29 and FLO-1 cells were measured using caspase 3/7 assay. Results: We found that intestinal epithelial cells with IFN-γ sensitization incubated with C. concisus significantly upregulated PD-L1 expression and significantly increased the production of interleukin (IL)-8. Whereas, PD-L1 expression was significantly inhibited in IFN-γ sensitized FLO-1 cells incubated with C. concisus strains. Furthermore, FLO-1 cells with and without IFN-γ sensitization incubated with C. concisus strains both had significantly higher levels of cell death. Conclusion: C. concisushas the potential to cause damage to both intestinal and esophageal epithelial cells, however, with different pathogenic effects.
摘要：
简介：弯曲杆菌是一种口腔细菌，与炎症性肠病（IBD）和Barrett食管（BE）有关。程序性细胞死亡配体-1（PD-L1）是一种免疫检查点蛋白，被肿瘤细胞用于免疫逃避，并在IBD和BE患者中表达增加。我们检查了C.concisus是否上调肠和食管上皮细胞中PD-L1的表达。方法：将有和没有干扰素（IFN）-γ致敏的人肠上皮HT-29细胞和食管上皮FLO-1细胞与C.concisus菌株孵育。使用定量实时PCR定量PD-L1mRNA的水平。使用酶联免疫吸附测定(ELISA)测量细胞因子。使用半胱天冬酶3/7测定法测量HT-29和FLO-1细胞的凋亡。结果：我们发现与C.concisus孵育的IFN-γ致敏的肠上皮细胞显着上调PD-L1表达并显着增加白介素（IL）-8的产生。然而,PD-L1表达在IFN-γ致敏的FLO-1细胞中被显著抑制。此外，与C.concisus菌株一起孵育的具有和不具有IFN-γ致敏的FLO-1细胞均具有显著更高水平的细胞死亡。结论：C.censushas可能会对肠道和食管上皮细胞造成损害，然而,具有不同的致病作用。
