PDF(Pubmed)
Abstract：
Cholangiopathies affect the biliary tree via various pathophysiological mechanisms. Research on biliary physiology and pathology, however, is hampered by a lack of physiologically relevant in vitro models. Conventional models, such as two-dimensional (2D) monolayers and organoids, fail to replicate the structural organization of the bile duct, and both the size of the duct and position of cells are difficult to manipulate in a controllable way. Here, we describe a bile duct-on-a-chip (BDOC) that phenocopies the open-ended tubular architecture of the bile duct in three dimensions which, when seeded with either a cholangiocyte cell line or primary cells, demonstrates barrier function similar to bile ducts in vivo. This device represents an in vitro platform to study the pathophysiology of the bile duct using cholangiocytes from a variety of sources.
摘要：
胆管病通过各种病理生理机制影响胆道树。胆道生理和病理研究，然而,由于缺乏生理相关的体外模型而受到阻碍。常规模型，例如二维（2D）单层和类器官，无法复制胆管的结构组织，管道的大小和细胞的位置都很难以可控的方式操纵。这里,我们描述了一种胆管芯片（BDOC），它在三维中表现出胆管的开放式管状结构，当接种胆管细胞系或原代细胞时，在体内表现出类似于胆管的屏障功能。该设备代表了使用来自多种来源的胆管细胞研究胆管病理生理学的体外平台。
