Abstract:
The simultaneous detection of cyanide (CN), thiocyanate (SCN), and selenocyanate (SeCN) by a HPLC-fluorescence detector (FLD) with the post-column König reaction was recently reported. SCN and SeCN are also detectable by HPLC-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) because sulfur and selenium can be detected, respectively, without any pre- or post-treatment. ICP-MS has high sensitivity for selenium and sulfur detection and is robust to sample matrices. In this study, we compared HPLC-FLD with the post-column König reaction and HPLC-ICP-MS in terms of SCN and SeCN detection sensitivity and linearity. The limit of detection (LOD) for SCN indicated that HPLC-FLD with the post-column König reaction was 354 times more sensitive than HPLC-ICP-MS. Likewise, the LOD for SeCN indicated that HPLC-FLD was 51 times more sensitive than HPLC-ICP-MS. These results demonstrated that HPLC-FLD was a more suitable technique for SeCN and SCN detection than HPLC-ICP-MS. We previously reported that SeCN was generated in selenite-exposed mammalian cells to detoxify excess selenite. HPLC-FLD with the post-column König reaction enabled good separation and detection for quantifying SCN and SeCN in mammalian cell lines exposed to selenite. The intracellular SCN and SeCN concentrations determined by this technique suggested differences in the metabolic capacity for selenite to form SeCN among the cell lines. In addition, since the amount of intracellular SCN and SeCN were significantly decreased by pretreatment of myeloperoxidase (MPO) inhibitors, SCN and SeCN were resulted from the interaction of sulfur and selenium with endogenous CN, respectively, generated with MPO.
摘要:
氰化物(CN)的同时检测,硫氰酸盐(SCN),最近报道了通过HPLC-荧光检测器(FLD)与柱后König反应的硒氰酸酯(SeCN)。SCN和SeCN也可以通过HPLC-电感耦合等离子体质谱法(HPLC-ICP-MS)检测到,因为可以检测到硫和硒,分别,没有任何前或后治疗。ICP-MS对硒和硫检测具有高灵敏度,并且对样品基质具有鲁棒性。在这项研究中,我们在SCN和SeCN检测灵敏度和线性方面比较了HPLC-FLD与柱后König反应和HPLC-ICP-MS。SCN的检测限(LOD)表明,柱后König反应的HPLC-FLD的灵敏度是HPLC-ICP-MS的354倍。同样,SeCN的LOD表明HPLC-FLD比HPLC-ICP-MS灵敏51倍。这些结果表明,HPLC-FLD比HPLC-ICP-MS更适合用于SeCN和SCN检测。我们先前报道了在暴露于亚硒酸盐的哺乳动物细胞中产生SeCN以对过量的亚硒酸盐进行解毒。具有柱后König反应的HPLC-FLD能够很好地分离和检测,以定量暴露于亚硒酸盐的哺乳动物细胞系中的SCN和SeCN。通过该技术确定的细胞内SCN和SeCN浓度表明,亚硒酸盐在细胞系中形成SeCN的代谢能力存在差异。此外,由于髓过氧化物酶(MPO)抑制剂的预处理显着降低了细胞内SCN和SeCN的量,SCN和SeCN是由硫和硒与内源CN相互作用产生的,分别,用MPO生成。