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Abstract：
Streptococcus suis is an emerging zoonotic bacterium causing septicemia and meningitis in humans. Due to rapid disease progression, high mortality rate, and many underdiagnosed cases by time-consuming routine identification methods, alternative diagnostic testing is essential. Among 29 broadly accepted S. suis serotypes, serotypes 2 and 14 are high prevalent; however, many PCR assays showed an inability to differentiate serotype 2 from 1/2, and 1 from 14. In this study, we developed and validated a new multiplex PCR assay that facilitates the identification of only the 29 true serotypes of S. suis and simultaneously differentiates serotypes 1, 1/2, 2, and 14 within a single reaction. Importantly, the multiplex PCR could detect S. suis directly from positive hemocultures and CSF. The results revealed high sensitivity, specificity, and 100% accuracy with almost perfect agreement (κ = 1.0) compared to culture and serotyping methods. Direct detection enables a decrease in overall diagnosis time, rapid and efficient treatment, reduced fatality rates, and proficient disease control. This multiplex PCR offers a rapid, easy, and cost-effective method that can be applied in a routine laboratory. Furthermore, it is promising for developing point-of-care testing (POCT) for S. suis detection in the future.
摘要：
猪链球菌是一种新兴的人畜共患细菌，可引起人类败血症和脑膜炎。由于疾病进展迅速，死亡率高,以及许多通过耗时的常规识别方法诊断不足的病例，替代诊断测试是必不可少的。在29种广泛接受的S.suis血清型中，血清型2和14很普遍；然而，许多PCR分析显示无法区分血清型2和1/2和1/14。在这项研究中,我们开发并验证了一种新的多重PCR检测方法,该方法仅有助于鉴定猪链球菌的29种真实血清型,并在单个反应中同时区分血清型1,1/2,2和14.重要的是,多重PCR可以直接从阳性血液培养物和CSF中检测猪链球菌。结果显示高灵敏度，特异性，与培养和血清分型方法相比，100％的准确性几乎完美一致（κ=1.0）。直接检测可以减少整体诊断时间，快速有效的治疗，降低死亡率，和熟练的疾病控制。这种多重PCR提供了一种快速、easy,和具有成本效益的方法，可应用于常规实验室。此外，它有望在将来开发用于猪链球菌检测的即时检测（POCT）。
