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Abstract：
Pesticides are extensively employed worldwide, especially in agriculture to control weeds, insect infestation and diseases. Besides their targets, pesticides can also affect the health of non-target organisms, including humans The present study was conducted to study the effect of oral exposure of thiram, a dithiocarbamate fungicide, on the intestine of rats. Male rats were administered thiram at doses of 100, 250, 500 and 750 mg/kg body weight for 4 days. This treatment reduced cellular glutathione, total sulfhydryl groups but enhanced protein carbonyl content and hydrogen peroxide levels. In addition, the activities of all major antioxidant enzymes (catalase, thioredoxin reductase, glutathione peroxidase and glutathione-S-transferase) except superoxide dismutase were decreased. The antioxidant power of the intestine was impaired lowering the metal-reducing and free radical quenching ability. Administration of thiram also led to inhibition of intestinal brush border membrane enzymes, alkaline phosphatase, γ-glutamyl transferase, leucine aminopeptidase and sucrase. Activities of enzymes of pentose phosphate pathway, citric acid cycle, glycolysis and gluconeogenesis were also inhibited. Histopathology showed extensive damage in the intestine of thiram-treated rats at higher doses. All the observed effects were in a thiram dose-dependent manner. The results of this study show that thiram causes significant oxidative damage in the rat intestine which is associated with the marked impairment in the antioxidant defense system.
摘要：
农药在全世界广泛使用，特别是在农业上控制杂草，昆虫侵扰和疾病。除了他们的目标,农药也会影响非目标生物的健康，包括人类本研究是为了研究口服硫美双的影响，一种二硫代氨基甲酸酯杀菌剂，在老鼠的肠道上。雄性大鼠以100、250、500和750mg/kg体重的剂量施用福美双4天。这种治疗减少了细胞谷胱甘肽，总巯基，但提高蛋白质羰基含量和过氧化氢水平。此外,所有主要抗氧化酶(过氧化氢酶，硫氧还蛋白还原酶，除超氧化物歧化酶外，谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶）均降低。肠道的抗氧化能力受损，降低了金属还原和自由基猝灭能力。福美双的给药还导致肠刷状缘膜酶的抑制，碱性磷酸酶,γ-谷氨酰转移酶，亮氨酸氨基肽酶和蔗糖酶。戊糖磷酸途径酶的活性,柠檬酸循环，糖酵解和糖异生也受到抑制。组织病理学显示，在较高剂量下，硫双治疗的大鼠的肠道中广泛损伤。所有观察到的效果都是以硫仑剂量依赖性方式。这项研究的结果表明，福美双在大鼠肠道中引起明显的氧化损伤，这与抗氧化防御系统的明显损伤有关。
