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Abstract：
BACKGROUND: Bergenia ciliata (Haw.) Sternb. is a plant growing in the Himalayan region of India where locals use its rhizomes for a variety of disease conditions including wounds and fractures. Although some of its pharmacological benefits have been documented, scientific validation of its wound healing property has not been done so far.
OBJECTIVE: To ensure use of this natural remedy as an alternative therapy to the faster wound healing, this study evaluated the wound healing activity of the ethanolic extract of Bergenia ciliata rhizome using excision wound model in Wistar rats.
METHODS: Four groups (n = 10) of rats were subjected to different topical wound regimens for 14 days. Simple paraffin-lanolin ointment was applied to the control group rats. One group was applied povidone-iodine 10% (w/w) ointment. The other two groups were treated with ointment of ethanolic extract of Bergenia ciliata at 5 or 10% (w/w) rhizome, respectively. Blood and wound tissue samples were collected on 7th and 14th day of treatment and were correspondingly subjected to histopathology, and the assays of L-hydroxyproline, D-glucosamine, antioxidants and pro-inflammatory cytokines.
RESULTS: Wound histology revealed increased collagenation, re-epithelialization and neovascularization while decreased bacterial colonies in the treatment groups. These histological changes and wound contraction were better in the 10% Bergenia ciliata group. Tissue L-hydroxyproline levels, blood enzymatic and non-enzymatic antioxidants were increased in the treatment groups. On 7th day of treatment glucosamine levels increased in the treatment groups, while as a reverse trend was observed on day 14. Plasma levels of TNF-α and IL-6 decreased in the treatment groups.
CONCLUSIONS: The results indicate that treatment with Bergenia ciliata extract ointment provides satisfactory wound healing which is comparable to that of the standard wound healing ointment, povidone-iodine and is surpassing simple lanolin-paraffin ointment. The improved wound healing, especially in the 10% Bergenia ciliata groups, can be attributed to satisfactory profile of the above studied parameters in these treatment groups which is also construed by the phytochemical analysis of its extract revealing the presence of antioxidant and anti-inflammatory compounds gallic acid, catechin, quercetin and rutin as the major active components.
OBJECTIVE: To ensure use of this natural remedy as an alternative therapy to the faster wound healing, this study evaluated the wound healing activity of the ethanolic extract of Bergenia ciliata rhizome using excision wound model in Wistar rats.
METHODS: Four groups (n = 10) of rats were subjected to different topical wound regimens for 14 days. Simple paraffin-lanolin ointment was applied to the control group rats. One group was applied povidone-iodine 10% (w/w) ointment. The other two groups were treated with ointment of ethanolic extract of Bergenia ciliata at 5 or 10% (w/w) rhizome, respectively. Blood and wound tissue samples were collected on 7th and 14th day of treatment and were correspondingly subjected to histopathology, and the assays of L-hydroxyproline, D-glucosamine, antioxidants and pro-inflammatory cytokines.
RESULTS: Wound histology revealed increased collagenation, re-epithelialization and neovascularization while decreased bacterial colonies in the treatment groups. These histological changes and wound contraction were better in the 10% Bergenia ciliata group. Tissue L-hydroxyproline levels, blood enzymatic and non-enzymatic antioxidants were increased in the treatment groups. On 7th day of treatment glucosamine levels increased in the treatment groups, while as a reverse trend was observed on day 14. Plasma levels of TNF-α and IL-6 decreased in the treatment groups.
CONCLUSIONS: The results indicate that treatment with Bergenia ciliata extract ointment provides satisfactory wound healing which is comparable to that of the standard wound healing ointment, povidone-iodine and is surpassing simple lanolin-paraffin ointment. The improved wound healing, especially in the 10% Bergenia ciliata groups, can be attributed to satisfactory profile of the above studied parameters in these treatment groups which is also construed by the phytochemical analysis of its extract revealing the presence of antioxidant and anti-inflammatory compounds gallic acid, catechin, quercetin and rutin as the major active components.
摘要：
背景：白菜属纤毛虫（Haw。)斯特恩布。是一种生长在印度喜马拉雅地区的植物，当地人将其根茎用于各种疾病，包括伤口和骨折。虽然它的一些药理益处已经被证明，到目前为止，尚未对其伤口愈合性能进行科学验证。
目的：为了确保使用这种自然疗法作为加速伤口愈合的替代疗法，本研究使用Wistar大鼠切除伤口模型评估了白菜花乙醇提取物的伤口愈合活性。
方法:对4组(n=10)大鼠进行不同的局部创伤方案14天。对照组大鼠应用石蜡-羊毛脂软膏。一组应用聚维酮碘10％（w/w）软膏。其他两组用5％或10％（w/w）根茎的白菜花乙醇提取物软膏治疗，分别。在治疗的第7天和第14天收集血液和伤口组织样本，并进行相应的组织病理学检查，和L-羟脯氨酸的测定，D-氨基葡萄糖,抗氧化剂和促炎细胞因子。
结果：伤口组织学显示胶原增多，治疗组的上皮再形成和新生血管形成，而细菌菌落减少。这些组织学变化和伤口收缩在10％的细毛群中更好。组织L-羟脯氨酸水平，治疗组的血液酶和非酶抗氧化剂增加.治疗第7天，治疗组葡萄糖胺水平升高，而在第14天观察到相反的趋势。治疗组血浆TNF-α和IL-6水平降低。
结论：结果表明，用纤毛虫提取物软膏治疗可提供令人满意的伤口愈合，与标准伤口愈合软膏相当，聚维酮碘和超越简单的羊毛脂石蜡软膏。伤口愈合的改善，特别是在10%的白菜属纤毛虫群中，可以归因于这些治疗组中上述研究参数的令人满意的概况，这也是通过其提取物的植物化学分析解释的，揭示了抗氧化剂和抗炎化合物没食子酸的存在，儿茶素,槲皮素和芦丁为主要活性成分。
目的：为了确保使用这种自然疗法作为加速伤口愈合的替代疗法，本研究使用Wistar大鼠切除伤口模型评估了白菜花乙醇提取物的伤口愈合活性。
方法:对4组(n=10)大鼠进行不同的局部创伤方案14天。对照组大鼠应用石蜡-羊毛脂软膏。一组应用聚维酮碘10％（w/w）软膏。其他两组用5％或10％（w/w）根茎的白菜花乙醇提取物软膏治疗，分别。在治疗的第7天和第14天收集血液和伤口组织样本，并进行相应的组织病理学检查，和L-羟脯氨酸的测定，D-氨基葡萄糖,抗氧化剂和促炎细胞因子。
结果：伤口组织学显示胶原增多，治疗组的上皮再形成和新生血管形成，而细菌菌落减少。这些组织学变化和伤口收缩在10％的细毛群中更好。组织L-羟脯氨酸水平，治疗组的血液酶和非酶抗氧化剂增加.治疗第7天，治疗组葡萄糖胺水平升高，而在第14天观察到相反的趋势。治疗组血浆TNF-α和IL-6水平降低。
结论：结果表明，用纤毛虫提取物软膏治疗可提供令人满意的伤口愈合，与标准伤口愈合软膏相当，聚维酮碘和超越简单的羊毛脂石蜡软膏。伤口愈合的改善，特别是在10%的白菜属纤毛虫群中，可以归因于这些治疗组中上述研究参数的令人满意的概况，这也是通过其提取物的植物化学分析解释的，揭示了抗氧化剂和抗炎化合物没食子酸的存在，儿茶素,槲皮素和芦丁为主要活性成分。
