METHODS: A double-blind, randomized, controlled trial was conducted. Ninety-three individuals who were initially diagnosed with dyslipidemia were randomly assigned to placebo or 40, 80, 160 or 320 mg/day anthocyanin groups. The supplementations were anthocyanin capsules (Medox, Norway). Platelet aggregation by light aggregometry of platelet-rich plasma, P-selectin, activated GPⅡbⅢa, reactive oxygen species (ROS), and mitochondrial membrane potential were tested at baseline, 6 weeks and 12 weeks.
RESULTS: Compared to placebo group, anthocyanins at 80 mg/day for 12 weeks reduced collagen-induced platelet aggregation (-3.39±2.36%) and activated GPⅡbⅢa (-8.25±2.45%) (P < 0.05). Moreover, compared to placebo group, anthocyanins at 320 mg/day inhibited collagen-induced platelet aggregation (-7.05±2.38%), ADP-induced platelet aggregation (-7.14±2.00%), platelet ROS levels (-14.55±1.86%), and mitochondrial membrane potential (7.40±1.56%) (P < 0.05). There were dose-response relationships between anthocyanins and the attenuation of platelet aggregation, mitochondrial membrane potential and ROS levels (P for trend <0.05). Furthermore, significantly positive correlations were observed between changes in collagen-induced (r = 0.473) or ADP-induced (r = 0.551) platelet aggregation and ROS levels in subjects with dyslipidemia after the 12-week intervention (P < 0.05).
CONCLUSIONS: Anthocyanin supplementation dose-dependently attenuates platelet function, and 12-week supplementation with 80 mg/day or more of anthocyanins can reduce platelet function in individuals with dyslipidemia.
BACKGROUND: None.
方法:双盲,随机化,进行了对照试验。最初被诊断为血脂异常的93人被随机分配到安慰剂组或40、80、160或320mg/天的花青素组。补充剂是花色苷胶囊(Medox,挪威)。通过富血小板血浆的光聚集法测量血小板聚集,P-选择素,激活的GPⅡbⅢa,活性氧(ROS),和线粒体膜电位在基线测试,6周和12周。
结果:与安慰剂组相比,花色苷在80mg/d下连续12周降低了胶原诱导的血小板聚集(-3.39±2.36%),并激活了GPⅡbⅢa(-8.25±2.45%)(P<0.05)。此外,与安慰剂组相比,320毫克/天的花色苷抑制胶原蛋白诱导的血小板聚集(-7.05±2.38%),ADP诱导的血小板聚集(-7.14±2.00%),血小板ROS水平(-14.55±1.86%),线粒体膜电位(7.40±1.56%)(P<0.05)。花青素与血小板聚集的衰减之间存在剂量-反应关系,线粒体膜电位和ROS水平(P<0.05)。此外,干预12周后,血脂异常患者胶原诱导(r=0.473)或ADP诱导(r=0.551)血小板聚集变化与ROS水平呈正相关(P<0.05)。
结论:补充花青素剂量依赖性地减弱血小板功能,12周补充80毫克/天或更多的花色苷可降低血脂异常个体的血小板功能。
背景:无。