关键词: 4E-BP1, 4E-binding protein 1 ADF, actin depolymerizing factors BOO, bladder outlet obstruction BPH, benign prostatic hyperplasia Bladder smooth muscle contraction CCK-8, Cell Counting Kit-8 Cofilin phosphorylation Ct, number of cycles DMSO, dimethyl sulfoxide EdU, 5-ethynyl-2′-deoxyuridine GAPDH, glyceraldehyde 3-phosphate dehydrogenase H&E, hematoxylin and eosin HBSMCs, human bladder smooth muscle cells HRP, horseradish peroxidase LIMK LIMKs, LIM kinases LUTS, lower urinary tract symptoms Lower urinary tract symptoms (LUTS) MLC, myosin light chain MW, molecular weight MYPT1, myosin-binding subunit OAB, overactive bladder Overactive bladder (OAB) PCNA, proliferating cell nuclear antigen RT-qPCR, reverse transcription and quantitative polymerase chain reaction STK16, serine/threonine kinase 16 TESK1, testicular protein kinase 1 TXA2, thromboxane A2 WST-8, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt siRNA, small interfering RNA
来 源:
DOI:10.1016/j.apsb.2021.01.005
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