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Abstract：
Cardiac neural crest cells arise in the caudal hindbrain and then migrate to the heart through the pharyngeal arches. These cells contribute to the formation of the heart, including the outflow tract, and are unique to this neural crest population. MafB is a transcription factor expressed specifically in early migrating cardiac neural crest cells as well as in rhombomeres (r) 5 and 6. Here, we identified the regulatory region in the chicken genome controlling the expression of endogenous MafB transcripts and used these essential elements to express MafB in the cardiac neural crest in reporter assays. A reporter driven by this regulatory region was employed to trace the migration of these cells into the pharyngeal arches. This regulatory region demonstrated transcriptional activity in the cardiac neural crest but not in other neural crest cell subpopulations, such as the cranial and trunk cells. This study provides insights into the gene regulatory mechanisms that specify cardiac neural crest cells among neural crest cell populations.
摘要：
心脏神经c细胞出现在尾后脑，然后通过咽弓迁移到心脏。这些细胞有助于心脏的形成，包括流出道，并且是这个神经脊群体所独有的。MafB是在早期迁移的心脏神经c细胞以及菱形（r）5和6中特异性表达的转录因子。这里,我们确定了鸡基因组中控制内源性MafB转录物表达的调控区,并在报告基因测定中使用这些必需元件在心脏神经c中表达MafB.由这个调控区驱动的报告子被用来追踪这些细胞迁移到咽弓中。该调控区在心脏神经c中表现出转录活性，但在其他神经c细胞亚群中却没有。例如颅骨和躯干细胞。这项研究提供了对神经c细胞群体中指定心脏神经c细胞的基因调控机制的见解。
