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Abstract：
Nowadays, the HIV pandemic is far from controlled. HIV+/AIDS patients show a serious risk of developing resistance to HIV antiretroviral drugs and to be orally colonized by albicans and non-albicans Candida strains resistant to antifungals. As a consequence, new drugs that possess anti-candidal and anti-HIV effects would represent an alternative in the comprehensive treatment of HIV+/AIDS patients. The present study evaluates the possible anti-HIV and anti-Candida effects of a methanolic extract from Heteropterys brachiata (Hb MeOH), an American tropical plant. The anti-HIV effect of Hb MeOH was tested using a non-radioactive colorimetric method (Lenti RT® Activity Assay; Cavidi Tech) that uses reverse transcriptase of HIV-1 enzyme as enzymatic target. The anti-candidal effect of HbMeOH extract was evaluated by following a standardized test protocol of microdilution for yeast using the Candida albicans strain ATCC® 90028. The Hb MeOH at 1 mg/mL concentration shows 38.5% RT-HIV inhibition, while Hb MeOH at 10 mg/mL concentration produced 98% C. albicans growth inhibition. Our findings show that the Hb MeOH possesses a strong anti-candidal activity and moderate anti-HIV effect and suggests that the plant extract could be considered as a potential candidate for HIV/AIDS treatment.
摘要：
如今,艾滋病毒的流行远未得到控制。HIV/AIDS患者显示出对HIV抗逆转录病毒药物产生抗性的严重风险，并被对抗真菌药物具有抗性的白色念珠菌和非白色念珠菌菌株口服定植。因此,具有抗念珠菌和抗艾滋病毒作用的新药将成为艾滋病毒/艾滋病患者综合治疗的替代方案。本研究评估了腕藻（HbMeOH）甲醇提取物的可能的抗HIV和抗念珠菌作用，一种美国热带植物。使用使用HIV-1酶的逆转录酶作为酶靶标的非放射性比色法(LentiRT®活性测定;CavidiTech)测试HbMeOH的抗HIV作用。通过遵循使用白色念珠菌菌株ATCC®90028的酵母微量稀释的标准化测试方案来评估HbMeOH提取物的抗念珠菌效果。1mg/mL浓度的HbMeOH显示38.5%RT-HIV抑制,而10mg/mL浓度的HbMeOH产生98%白色念珠菌生长抑制。我们的发现表明，HbMeOH具有很强的抗念珠菌活性和中等的抗HIV作用，并表明植物提取物可被视为HIV/AIDS治疗的潜在候选物。
