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Abstract：
Immune cells are generated from hematopoietic stem cells (HSCs) in the bone marrow (BM). Immune stimulation can rapidly activate HSCs out of their quiescent state to accelerate the generation of immune cells. HSCs\' activation follows various viral or bacterial stimuli, and we sought to investigate the hypersensitivity immune response. Surprisingly, the Ova-induced hypersensitivity peritonitis model finds no significant changes in BM HSCs. HSC markers cKIT, SCA1, CD48, CD150, and the Fgd5-mCherry reporter showed no significant difference from control. Functionally, hypersensitivity did not alter HSCs\' potency, as assayed by transplantation. We further characterized the possible impact of hypersensitivity using RNA-sequencing of HSCs, finding minor changes at the transcriptome level. Moreover, hypersensitivity induced no significant change in the proliferative state of HSCs. Therefore, this study suggests that, in contrast to other immune stimuli, hypersensitivity has no impact on HSCs.
摘要：
免疫细胞由骨髓(BM)中的造血干细胞(HSC)产生。免疫刺激可以快速激活HSC脱离其静止状态以加速免疫细胞的生成。HSC活化遵循各种病毒或细菌刺激，我们试图调查超敏反应的免疫反应。令人惊讶的是,Ova诱导的超敏反应性腹膜炎模型在BMHSC中没有发现显著变化。HSC标记cKIT,SCA1、CD48、CD150和Fgd5-mCherry报道分子与对照没有显著差异。功能上,超敏反应不会改变HSC的效力，通过移植测定。我们使用HSC的RNA测序进一步表征了超敏反应的可能影响，在转录组水平发现微小的变化。此外，超敏反应对HSC的增殖状态没有明显的改变。因此,这项研究表明，与其他免疫刺激相反，超敏反应对HSC没有影响。
