Abstract：
BACKGROUND: To compare the number of lymph nodes (LNs) detected when using Carnoy\'s solution (CS) versus 10% neutral buffered formalin (NBF) to fix specimens after radical gastrectomy for gastric cancer.
METHODS: LNs were routinely detected using NBF until 2020, since then, for the fixation procedure, residual fat was fixed in CS for 24 hours and dissected again for the detection of further LNs. Of 143 specimens, 117 were included in the NBF group and 26 in the CS group.
RESULTS: The mean numbers of LNs examined were 27.85±14.89 and 36.30±12.41 in the NBF and CS groups, respectively (p=0.008). The mean number of additional LNs detected using CS was 8.07±2.91, of which 0.38±1.02 were metastatic. Additional LNs were found in all patients of the CS group, and all were ≤3 mm. Of the 26 patients in the CS group, metastatic LNs were detected in four, disease in two of whom was up-staged.
CONCLUSIONS: CS is an appropriate alternative to NBF for the fixation of gastric cancer specimens, and more LNs were detected in the resected specimens fixed when using CS compared with NBF.
METHODS: LNs were routinely detected using NBF until 2020, since then, for the fixation procedure, residual fat was fixed in CS for 24 hours and dissected again for the detection of further LNs. Of 143 specimens, 117 were included in the NBF group and 26 in the CS group.
RESULTS: The mean numbers of LNs examined were 27.85±14.89 and 36.30±12.41 in the NBF and CS groups, respectively (p=0.008). The mean number of additional LNs detected using CS was 8.07±2.91, of which 0.38±1.02 were metastatic. Additional LNs were found in all patients of the CS group, and all were ≤3 mm. Of the 26 patients in the CS group, metastatic LNs were detected in four, disease in two of whom was up-staged.
CONCLUSIONS: CS is an appropriate alternative to NBF for the fixation of gastric cancer specimens, and more LNs were detected in the resected specimens fixed when using CS compared with NBF.
摘要：
背景：比较胃癌根治术后使用Carnoy's溶液（CS）与10％中性缓冲福尔马林（NBF）固定标本时检测到的淋巴结（LN）数量。
方法：在2020年之前，使用NBF常规检测LN，此后，对于固定程序，将残余脂肪在CS中固定24小时并再次解剖以检测进一步的LN。在143个样本中，NBF组包含117个，CS组包含26个。
结果：在NBF和CS组中，检查的LN的平均数量为27.85±14.89和36.30±12.41，分别(p=0.008)。使用CS检测到的其他LN的平均数量为8.07±2.91，其中0.38±1.02是转移性的。在CS组的所有患者中发现了额外的LN，且均≤3mm。在CS组的26名患者中，在四个中检测到转移性LN，其中两个人的疾病被升级。
结论：CS是用于固定胃癌标本的NBF的合适替代方法，与NBF相比，使用CS固定的切除标本中检测到更多的LN。
方法：在2020年之前，使用NBF常规检测LN，此后，对于固定程序，将残余脂肪在CS中固定24小时并再次解剖以检测进一步的LN。在143个样本中，NBF组包含117个，CS组包含26个。
结果：在NBF和CS组中，检查的LN的平均数量为27.85±14.89和36.30±12.41，分别(p=0.008)。使用CS检测到的其他LN的平均数量为8.07±2.91，其中0.38±1.02是转移性的。在CS组的所有患者中发现了额外的LN，且均≤3mm。在CS组的26名患者中，在四个中检测到转移性LN，其中两个人的疾病被升级。
结论：CS是用于固定胃癌标本的NBF的合适替代方法，与NBF相比，使用CS固定的切除标本中检测到更多的LN。
