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Abstract：
Bone hydrolysates from bighead carp (Aristichthys nobilis) were prepared using Protamex and Alcalase with degrees of hydrolysis (DH) of 5%, 10% and 15%. The antioxidant activity of bone hydrolysates was evaluated in vitro and then the hydrolysates with better antioxidant activity were used to immerse bighead carp fillets through a vacuum impregnation process at concentrations of 1% and 2%. Among the six hydrolysates, fish bone hydrolyzed with Protamex at DH 10% exhibited the highest ability to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) (88.79%), 2, 2\'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) (57.76%) and hydroxyl radicals (62.72%), as well as to chelate ferrous ions (91.46%). The hydrolysates effectively postponed freezing- and thawing-induced protein/lipid oxidation. Compared with the fillets without treatment, the impregnated fillets had higher sulfhydryl contents, greater Ca2+-ATPase activity, lower carbonyls and lower thiobarbituric acid-reactive substances (TBARS). Bone hydrolysates also have a positive effect on the texture and water-holding ability of freeze-thawed fish fillets. Fish bone hydrolysates of Protamex could serve as potential antioxidants to preserve fish fillets.
摘要：
使用Protamex和Alcalase制备Bighe鱼（Aristichthysnobilis）的骨水解产物，水解度（DH）为5％，10%和15%。在体外评估了骨水解物的抗氧化活性，然后将具有更好抗氧化活性的水解物用于通过真空浸渍工艺以1％和2％的浓度浸入big鱼片。在六种水解产物中，用Protamex水解的鱼骨在DH10％时表现出最高的清除1，1-二苯基-2-吡啶酰肼（DPPH）的能力（88.79％），2，2'-偶氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）（57.76％）和羟基（62.72％），以及螯合亚铁离子(91.46%)。水解产物有效地延缓了冻结和解冻诱导的蛋白质/脂质氧化。与未经处理的鱼片相比，浸渍的鱼片具有较高的巯基含量，更高的Ca2+-ATPase活性，低级羰基和低级硫代巴比妥酸反应性物质（TBARS）。骨水解产物对冻融鱼片的质地和保水能力也有积极影响。Protamex的鱼骨水解产物可以作为潜在的抗氧化剂来保存鱼片。
