Two Intercalation Mechanisms of Oxazole Yellow Dimer (YOYO-1) into DNA.

• 影响指数: 4.927
• 发表时间: Jun 2021 19
• 来源期刊: Molecules
• DOI: 10.3390/molecules26123748
• PMID: 34205435
• 文章类型: Journal Article
• 中科院分区: Q3
• 作者列表: Kucharska K,Pilz M,Bielec K,Kalwarczyk T,Kuźma P,Hołyst R

关键词: DNA; YOYO-1; brightness method; equilibrium constant; intercalation

Mesh: Benzoxazoles / chemistry; DNA / chemistry; Dimerization; Fluorescent Dyes / chemistry; Intercalating Agents / chemistry; Quinolines / chemistry; Quinolinium Compounds / chemistry

Abstract

The oxazole yellow dye, YOYO-1 (a symmetric homodimer), is a commonly used molecule for staining DNA. We applied the brightness analysis to study the intercalation of YOYO-1 into the DNA. We distinguished two binding modes of the dye to dsDNA: mono-intercalation and bis-intercalation. Bis-intercalation consists of two consecutive mono-intercalation steps, characterised by two distinct equilibrium constants (with the average number of base pair per binding site equals 3.5): K1=3.36±0.43×107M-1 and K2=1.90±0.61×105M-1, respectively. Mono-intercalation dominates at high concentrations of YOYO-1. Bis-intercalation occurs at low concentrations.