{Reference Type}: Journal Article
{Title}: Two Intercalation Mechanisms of Oxazole Yellow Dimer (YOYO-1) into DNA.
{Author}: Kucharska K;Pilz M;Bielec K;Kalwarczyk T;Kuźma P;Hołyst R;
{Journal}: Molecules
{Volume}: 26
{Issue}: 12
{Year}: Jun 2021 19
{Factor}: 4.927
{DOI}: 10.3390/molecules26123748
{Abstract}: The oxazole yellow dye, YOYO-1 (a symmetric homodimer), is a commonly used molecule for staining DNA. We applied the brightness analysis to study the intercalation of YOYO-1 into the DNA. We distinguished two binding modes of the dye to dsDNA: mono-intercalation and bis-intercalation. Bis-intercalation consists of two consecutive mono-intercalation steps, characterised by two distinct equilibrium constants (with the average number of base pair per binding site equals 3.5): K1=3.36±0.43×107M-1 and K2=1.90±0.61×105M-1, respectively. Mono-intercalation dominates at high concentrations of YOYO-1. Bis-intercalation occurs at low concentrations.