研究翻译假基因的方法 : 重组表达和互补 ,靶向蛋白质组学,和 RNA 分析。
关键词: Cloning DHFR Expression proteomics Pseudogene RNA profiling
Mesh : Animals Cell Line Chromatography, Liquid / methods Cloning, Molecular / methods DNA Probes Escherichia coli Genetic Vectors / genetics Humans Mammals Open Reading Frames / genetics Protein Biosynthesis Proteomics / methods Pseudogenes / genetics RNA, Messenger / genetics Recombinant Fusion Proteins / genetics isolation & purification metabolism Reverse Transcriptase Polymerase Chain Reaction / methods Subcellular Fractions / chemistry Tandem Mass Spectrometry / methods
来 源: DOI:10.1007/978-1-0716-1503-4_15