Abstract：
Long intergenic noncoding RNAs (lincRNAs) are known to be tissue specifically expressed and able to regulate functional protein-coding genes: some can even act as competing endogenous RNAs (ceRNAs), because microRNAs can bind to them instead of the corresponding mRNA binding sites. Some lincRNAs contain remnants of protein-coding sequences and it has been hypothesized that they might arise after a pseudogenization processes. However, a major limitation in the study of such phenomenon is the lack of proper computational tools designed to align/analyze protein-coding sequences and noncoding sequences. To overcome this limitation, we published a method that finds the remnants of protein-coding sequences within the sequence of lincRNAs, as well as the corresponding sequences in parental proteins. This method, together with the visualization platform for tracing frameshifts and single point mutations within this type of sequences, are described here.
摘要：
长基因间非编码RNA（lincRNAs）已知是组织特异性表达的，能够调节功能性蛋白质编码基因：有些甚至可以充当竞争内源性RNA（ceRNAs）。因为microRNA可以与它们结合，而不是相应的mRNA结合位点。一些lincRNAs含有蛋白质编码序列的残留物，并且已经假设它们可能在伪发生过程之后出现。然而,研究这种现象的一个主要限制是缺乏用于比对/分析蛋白质编码序列和非编码序列的适当计算工具。为了克服这个限制,我们发表了一种方法，在lincRNAs序列中找到蛋白质编码序列的残留物，以及亲本蛋白质中的相应序列。这种方法,以及用于跟踪此类序列中的移码和单点突变的可视化平台，在这里描述。
