Abstract：
Epidermophyton floccosum is one of the most common agents of human superficial fungal infections, compared with genus Trichophyton and Microsporum, it possesses uniqueness in ecology traits and rarely causing hair infections. E. floccosum is so far the only representative species of genera Epidermophyton, and it is known as anthropophilic dermatophytes. To further reveal the genome sequences and clues of virulence factors, thus in this study, we sequenced the genome of E. floccosum (CGMCC (F) E1d), and performed comparative genomic analysis with other dermatophytes. It is revealed that E. floccosum owns the largest genome size and similar GC content compared with other dermatophytes. A total of 7565 genes are predicted. By comparing with the closest species N. gypseum, our study reveals that number and structure of adhesion factors, secreted proteases and LysM domain might contribute to the pathogenic and ecological traits of E. floccosum. Mating genes is also detected in genome data. Furthermore, we performed AFLP analysis trying to discuss intraspecific differences of E. floccosum, but no significant relationship is found between genotype and geographical distribution. Upon above, our study provides a deeper understanding and strong foundation for future researches about E. floccosum.
摘要：
絮状表皮生长因子是人类浅表真菌感染的最常见因子之一，与毛癣菌属和小孢子菌属相比，它具有独特的生态特征，很少引起头发感染。到目前为止，絮状大肠杆菌是表皮植物属的唯一代表性物种，它被称为嗜人性皮肤癣菌。为了进一步揭示毒力因子的基因组序列和线索，因此，在这项研究中，我们对絮状芽孢杆菌(CGMCC(F)E1d)的基因组进行了测序，并与其他皮肤癣菌进行比较基因组分析。结果表明，与其他皮肤癣菌相比，絮凝剂具有最大的基因组大小和相似的GC含量。预测了总共7565个基因。通过与最接近的石膏N.物种进行比较，我们的研究揭示了粘附因子的数量和结构，分泌的蛋白酶和LysM结构域可能与絮凝大肠杆菌的致病和生态特性有关。在基因组数据中也检测到交配基因。此外，我们进行了AFLP分析，试图讨论絮凝剂的种内差异，但基因型与地理分布之间没有显著关系。在上面，我们的研究为今后对絮凝剂的研究提供了更深入的理解和坚实的基础。
