PDF(Sci-hub)
Abstract：
Liamocins synthesized by Aureobasidium spp. are glycolipids composed of a single mannitol or arabitol headgroup linked to either three, four or even six 3,5-dihydroxydecanoic ester tail-groups. The highest titer of liamocin achieved was over 40.0 g/L. The substrates for liamocins synthesis include glucose, sucrose, xylose, mannitol, and others. The Pks1 is responsible for the biosynthesis of the tail-group 3,5-dihydroxydecanoic acid, both mannitol dehydrogenase (MDH) and mannitol 1-phosphate 5-dehydrogenase (MPDH) catalyze the mannitol biosynthesis and the arabitol biosynthesis is controlled by arabitol dehydrogenase (ArDH). The ester bond formation between 3,5-dihydroxydecanoic acid and mannitol or arabitol is catalyzed by the esterase (Est1). Liamocin biosynthesis is regulated by the specific transcriptional activator (Gal1), global transcriptional activator (Msn2), various signaling pathways, acetyl-CoA flux while Pks1 activity is controlled by PPTase activity. The synthesized liamocins have high bioactivity against the pathogenic bacteria Streptococcus spp. and some kinds of cancer cells while Massoia lactone released liamocins which exhibited obvious antifungal and anticancer activities. Therefore, liamocins and Massoia lactone have many applications in various sectors of biotechnology.
摘要：
金黄色葡萄球菌合成的Liamocins。糖脂由单个甘露醇或阿拉伯糖醇头基组成，四个甚至六个3,5-二羟基癸酸酯尾基。获得的最高滴度超过40.0g/L。用于合成的底物包括葡萄糖，蔗糖,木糖,甘露醇,和其他人。Pks1负责尾基3,5-二羟基癸酸的生物合成，甘露醇脱氢酶(MDH)和甘露醇1-磷酸5-脱氢酶(MPDH)催化甘露醇生物合成,并且阿拉伯糖醇生物合成由阿拉伯糖醇脱氢酶(ArDH)控制。3,5-二羟基癸酸与甘露醇或阿拉伯糖醇之间的酯键形成由酯酶(Est1)催化。Liamocin的生物合成受特定转录激活因子（Gal1）的调节，全局转录激活因子(Msn2)，各种信号通路，乙酰辅酶A通量，而Pks1活性受PPTase活性控制。合成的Liamocin对病原菌链球菌具有很高的生物活性。和某些种类的癌细胞，而马苏木内酯释放的金属霉素具有明显的抗真菌和抗癌活性。因此,在生物技术的各个领域中，有许多应用。
