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Abstract：
OBJECTIVE: Two putative probiotic strains, Lacticaseibacillus (Lc.) rhamnosus BFE5264 and Lactiplantibacillus (Lp.) plantarum NR74, have been shown to suppress cholesterol uptake and promote cholesterol efflux in Caco-2 cells. However, an in vivo beneficial effect of these strains on plasma cholesterol levels has not been verified yet; neither have the underlying mechanisms of regulating cholesterol metabolism clarified thus far. This study has focused on these two aspects.
RESULTS: A murine model has been used, and the animals receiving a high-fat/high-cholesterol diet showed elevated plasma cholesterol levels. However, supplementation of Lc. rhamnosus BFE5264 and Lp. plantarum NR74 resulted in the down regulation of Niemann-Pick C1-like 1 (NPC1L1) in the intestine in addition to counteracting the diet-induced suppression of low-density lipoprotein receptor expression in the liver. ATP Binding Cassette Subfamily A Member 1 (ABCA1) was only significantly increased upon administration of Lc. rhamnosus BFE5264.
CONCLUSIONS: The present findings demonstrate that supplementation with Lc. rhamnosus BFE5264 and Lp. plantarum NR74 may improve diet-induced hypercholesterolemia by suppression of cholesterol absorption in the small intestine and by supporting the regulation of cholesterol metabolism in the liver.
CONCLUSIONS: This work contributes to understanding the beneficial effects of probiotics on host cholesterol metabolism and underlying mechanisms related to hypercholesterolemia.
RESULTS: A murine model has been used, and the animals receiving a high-fat/high-cholesterol diet showed elevated plasma cholesterol levels. However, supplementation of Lc. rhamnosus BFE5264 and Lp. plantarum NR74 resulted in the down regulation of Niemann-Pick C1-like 1 (NPC1L1) in the intestine in addition to counteracting the diet-induced suppression of low-density lipoprotein receptor expression in the liver. ATP Binding Cassette Subfamily A Member 1 (ABCA1) was only significantly increased upon administration of Lc. rhamnosus BFE5264.
CONCLUSIONS: The present findings demonstrate that supplementation with Lc. rhamnosus BFE5264 and Lp. plantarum NR74 may improve diet-induced hypercholesterolemia by suppression of cholesterol absorption in the small intestine and by supporting the regulation of cholesterol metabolism in the liver.
CONCLUSIONS: This work contributes to understanding the beneficial effects of probiotics on host cholesterol metabolism and underlying mechanisms related to hypercholesterolemia.
摘要：
目的：两种推定的益生菌菌株，乳杆菌（Lc。)鼠李糖BFE5264和Lactiplantibacillus(Lp。）植物乳NR74已被证明可以抑制Caco-2细胞中的胆固醇摄取并促进胆固醇流出。然而,这些菌株对血浆胆固醇水平的体内有益作用尚未得到证实；迄今为止，调节胆固醇代谢的潜在机制也没有得到澄清。本研究主要集中在这两个方面。
结果：已经使用了一个小鼠模型，接受高脂肪/高胆固醇饮食的动物显示血浆胆固醇水平升高。然而,补充Lc。鼠李糖BFE5264和Lp。除了抵消饮食诱导的肝脏低密度脂蛋白受体表达抑制外，植物植物NR74还导致肠道中Niemann-PickC1样1（NPC1L1）的下调。ATP结合盒亚家族A成员1(ABCA1)仅在施用Lc时显著增加。鼠李糖BFE5264.
结论：本研究结果表明补充Lc。鼠李糖BFE5264和Lp。足底NR74可能通过抑制小肠中胆固醇的吸收和支持肝脏中胆固醇代谢的调节来改善饮食诱导的高胆固醇血症.
结论：这项工作有助于了解益生菌对宿主胆固醇代谢的有益作用以及与高胆固醇血症相关的潜在机制。
结果：已经使用了一个小鼠模型，接受高脂肪/高胆固醇饮食的动物显示血浆胆固醇水平升高。然而,补充Lc。鼠李糖BFE5264和Lp。除了抵消饮食诱导的肝脏低密度脂蛋白受体表达抑制外，植物植物NR74还导致肠道中Niemann-PickC1样1（NPC1L1）的下调。ATP结合盒亚家族A成员1(ABCA1)仅在施用Lc时显著增加。鼠李糖BFE5264.
结论：本研究结果表明补充Lc。鼠李糖BFE5264和Lp。足底NR74可能通过抑制小肠中胆固醇的吸收和支持肝脏中胆固醇代谢的调节来改善饮食诱导的高胆固醇血症.
结论：这项工作有助于了解益生菌对宿主胆固醇代谢的有益作用以及与高胆固醇血症相关的潜在机制。
