PDF(Sci-hub)
Abstract：
Cameline filarosis is an important parasitic disease having an economic impact on the camel industry around the world. However, there has been no study on filarosis in Bactrian camels of Mongolia. Therefore, the aim of the present study was to detect and identify microfilariae of Deraiophoronema evansi (D. evansi) in Bactrian camels from three provinces, located in southern and southwestern Mongolia. Blood samples were obtained from 400 healthy two-humped camels of different ages and both sexes. All blood samples were analysed using a variety of diagnostic techniques. Microfilariae were detected in 30 Bactrian camels (7.5%) by the Knott technique, while 13 Bactrian camels (3.3%) tested positive in a direct smear test. D. evansi was detected in 18 Bactrian camels (4.5%) by PCR assay. Prevalence was shown to be high among Bactrian camels in the age group up to 5 years, while the lowest positive results were obtained for Bactrian camels in the 5-10-year age group and the over 10-year age group. To confirm the morphological identification, D. evansi-COI gene sequences were subjected to phylogenetic analyses. The D. evansi-COI gene sequences from Mongolian two-humped camels were identical to sequences from Iranian one-humped camels and were clustered together with these sequences in the phylogeny. This is the first report of molecular detection and identification of microfilariae of D. evansi in Bactrian camels of Mongolia.
摘要：
驼线丝虫病是一种重要的寄生虫病，对世界各地的骆驼产业产生经济影响。然而,没有关于蒙古双峰骆驼丝虫病的研究。因此,本研究的目的是检测和鉴定evansi的微丝虫（D.evansi）来自三个省的双峰驼，位于蒙古南部和西南部。从400只不同年龄和两种性别的健康双峰骆驼中获得血液样本。使用多种诊断技术分析所有血液样品。通过Knott技术在30只双峰骆驼（7.5％）中检测到微丝虫，而13只双峰驼(3.3%)在直接涂片试验中呈阳性。通过PCR检测18只双峰骆驼（4.5％）中的伊凡氏D。在5岁以下年龄段的双峰骆驼中，患病率很高，而双峰骆驼在5-10岁年龄组和10岁以上年龄组的阳性结果最低。为了确认形态鉴定，对D.evansi-COI基因序列进行系统发育分析。蒙古双峰骆驼的D.evansi-COI基因序列与伊朗单峰骆驼的序列相同，并在系统发育中与这些序列聚集在一起。这是蒙古双峰骆驼中伊万氏微丝虫的分子检测和鉴定的首次报道。
