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Abstract：
Anandamide (AEA) is one of the best characterized members of the endocannabinoid family and its involvement in many pathophysiological processes has been well documented in vertebrates and invertebrates. Here, we report the biochemical and functional characterization of key elements of the endocannabinoid system in hemocytes isolated from the Mediterranean mussel Mytilus galloprovincialis. We also show the effects of exogenous AEA, as well as of capsaicin, on the cell ability to migrate and to activate the respiratory burst, upon in vitro stimulation of phagocytosis. Interestingly, our findings show that both AEA and capsaicin suppress the hemocyte response and that the use of selective antagonists of CB2 and TRPV1 receptors revert their inhibitory effects. Overall, present data support previous evidence on the presence of endocannabinoid signaling in mollusks and advance our knowledge about the evolutionary origins of this endogenous system and its role in the innate response of mollusks.
摘要：
Anandamide（AEA）是内源性大麻素家族中特征最好的成员之一，其参与许多病理生理过程已在脊椎动物和无脊椎动物中得到充分证明。这里,我们报告了从地中海贻贝Mytilusgalloprovincialis分离的血细胞中内源性大麻素系统关键元件的生化和功能特征。我们还展示了外源AEA的影响，以及辣椒素，细胞迁移和激活呼吸爆发的能力，在体外刺激吞噬作用。有趣的是,我们的研究结果表明,AEA和辣椒素均能抑制血细胞反应,使用CB2和TRPV1受体的选择性拮抗剂可恢复其抑制作用.总的来说,目前的数据支持先前关于软体动物中存在内源性大麻素信号的证据，并增进了我们对这种内源性系统的进化起源及其在软体动物先天反应中的作用的认识。
