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Abstract：
To study reproductive toxicity of gene modified wheat generated by introducing DREB3 (drought response element binding protein 3) gene, Wistar rats of were allocated into 3 groups and fed with DREB3 gene modified wheat mixture diet (GM group), non-gene modified wheat mixture diet (Non-GM group) and AIN-93 diet (Control group) from parental generation (F0) to the second offspring (F2). GM wheat and Non-GM wheat, Jimai22, were both formulated into diets at a ratio of 69.55% according to AIN93 diet for rodent animals. Compared with non-GM group, no biologically related differences were observed in GM group rats with respect to reproductive performance such as fertility rate, gestation rate, mean duration, hormone level, reproductive organ pathology and developmental parameters such as body weight, body length, food consumption, neuropathy, behavior, immunotoxicity, hematology and serum chemistry. In conclusion, no adverse effect were found relevant to GM wheat in the two generation reproduction toxicity study, indicating the GM wheat is a safe alternative for its counterpart wheat regarding to reproduction toxicity.
摘要：
研究通过引入DREB3（干旱响应元件结合蛋白3）基因产生的转基因小麦的生殖毒性。将Wistar大鼠分为3组，饲喂DREB3基因修饰的小麦混合饮食（GM组），从亲本世代（F0）到第二个后代（F2），非基因修饰的小麦混合饮食（非GM组）和AIN-93饮食（对照组）。转基因小麦和非转基因小麦，根据针对啮齿动物的AIN93饮食，Jimai22均以69.55％的比例配制成饮食。与非GM组相比,没有生物学相关的差异观察到在GM组大鼠的生殖性能，如生育率，妊娠率,平均持续时间，激素水平,生殖器官病理学和发育参数，如体重，身体长度,食物消费,神经病,行为，免疫毒性,血液学和血清化学。总之,在两代生殖毒性研究中，没有发现与转基因小麦相关的不良影响，表明转基因小麦在生殖毒性方面是其对应小麦的安全替代品。
