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Abstract：
The review article is dedicated to a comprehensive study of the chemical bond formed during the immobilization of the proteolytic enzyme pancreatic trypsin in chitosan-based polymer matrixes and its derivatives. The main focus of the study is to describe the chemical bond that causes immobilization between chitosan based carriers and trypsin. Because the nature of the chemical bond between the carrier and trypsin is a key factor in determining the area of application of the conjugate. It has been found out that after the chemical nature of functional groups, their degree of ionization, the structure of the chemical cross-linking, the medium pH and ionic strength of chitosan are modified, the mechanism of trypsin immobilization is affected. As a result, the attraction enzyme to the matrix occurs due to polar covalent and hydrogen bonds, as well as electrostatic, hydrophobic, Van der Waals forces. The collected research works on the immobilization of trypsin on chitosan-based carriers have been systematized in the paper and shown schematically in subsystems according to the type of chemical interaction. It has been shown that the immobilization of trypsin on chitosan based matrixes occur more often due to the covalent and hydrogen bonds between the protein and the carrier.
摘要：
评论文章致力于全面研究蛋白水解酶胰腺胰蛋白酶在壳聚糖基聚合物基质及其衍生物中固定过程中形成的化学键。研究的主要重点是描述导致基于壳聚糖的载体和胰蛋白酶之间固定的化学键。因为载体和胰蛋白酶之间的化学键的性质是决定缀合物的应用领域的关键因素。已经发现，在官能团的化学性质之后，它们的电离程度，化学交联的结构，改性壳聚糖的介质pH值和离子强度,胰蛋白酶固定化的机制受到影响。因此,对基质的吸引酶是由于极性共价键和氢键而发生的，以及静电，疏水，范德华部队.所收集的关于将胰蛋白酶固定在壳聚糖基载体上的研究工作已在论文中系统化，并根据化学相互作用的类型在子系统中进行了示意性显示。已经表明，由于蛋白质和载体之间的共价键和氢键，胰蛋白酶在基于壳聚糖的基质上的固定更经常发生。
