Abstract：
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discussed in this chapter, and optimized silver staining protocols are proposed.
摘要：
银染用于在聚丙烯酰胺凝胶上电泳分离后检测蛋白质。它结合了出色的灵敏度（在低纳克范围内）与使用非常简单和廉价的设备和化学品。因为它在蛋白质组学中的应用，必须考虑两个重要的附加功能，与质谱和定量响应的兼容性。本章将讨论这两个功能，并提出了优化的银染方案。
