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Abstract：
The tubulin cytoskeleton plays an important role in establishing legume-rhizobial symbiosis at all stages of its development. Previously, tubulin cytoskeleton organization was studied in detail in the indeterminate nodules of two legume species, Pisum sativum and Medicago truncatula. General as well as species-specific patterns were revealed. To further the understanding of the formation of general and species-specific microtubule patterns in indeterminate nodules, the tubulin cytoskeleton organization was studied in three legume species (Vicia sativa, Galega orientalis, and Cicer arietinum). It is shown that these species differ in the shape and size of rhizobial cells (bacteroids). Immunolocalization of microtubules revealed the universality of cortical and endoplasmic microtubule organization in the meristematic cells, infected cells of the infection zone, and uninfected cells in nodules of the three species. However, there are differences in the endoplasmic microtubule organization in nitrogen-fixing cells among the species, as confirmed by quantitative analysis. It appears that the differences are linked to bacteroid morphology (both shape and size).
摘要：
微管蛋白细胞骨架在其发育的各个阶段建立豆科植物-根瘤菌共生中起着重要作用。以前,在两种豆科植物的不确定结节中详细研究了微管蛋白细胞骨架组织，紫花苜蓿和硬紫花苜蓿。揭示了一般和特定物种的模式。为了进一步了解不确定结核中一般和特定物种的微管模式的形成，在三种豆科植物中研究了微管蛋白细胞骨架组织(Viciasativa，GalegaOrientalis,和Cicerarietinum）。结果表明，这些物种的根瘤菌细胞（类细菌）的形状和大小不同。微管的免疫定位揭示了分生组织细胞中皮质和内质微管组织的普遍性，感染区的感染细胞，和这三个物种的结节中未感染的细胞。然而,固氮细胞的内质微管组织在物种之间存在差异，经定量分析证实。似乎差异与类细菌形态(形状和大小)有关。
