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Abstract：
Direct pulp capping is a vital pulp therapy for a pin-point dental pulp exposure. Applying a pulp capping material leads to the formation of a dentin bridge and protects pulp vitality. The aim of this study was to compare the effects of four dental materials, DyCal®, ProRoot® MTA, Biodentine™, and TheraCal™ LC in vitro.
Human dental pulp stem cells (hDPs) were isolated and characterized. Extraction medium was prepared from the different pulp capping materials. The hDP cytotoxicity, proliferation, and migration were examined. The odonto/osteogenic differentiation was determined by alkaline phosphatase, Von Kossa, and alizarin red s staining. Osteogenic marker gene expression was evaluated using real-time polymerase chain reaction.
ProRoot® MTA and Biodentine™ generated less cytotoxicity than DyCal® and TheraCal™ LC, which were highly toxic. The hDPs proliferated when cultured with the ProRoot® MTA and Biodentine™ extraction media. The ProRoot® MTA and Biodentine™ extraction medium induced greater cell attachment and spreading. Moreover, the hDPs cultured in the ProRoot® MTA or Biodentine™ extraction medium migrated in a similar manner to those in serum-free medium, while a marked reduction in cell migration was observed in the cells cultured in DyCal® and TheraCal™ LC extraction media. Improved mineralization was detected in hDPs maintained in ProRoot® MTA or Biodentine™ extraction medium compared with those in serum-free medium.
This study demonstrates the favorable in vitro biocompatibility and bioactive properties of ProRoot® MTA and Biodentine™ on hDPs, suggesting their superior regenerative potential compared with DyCal® and TheraCal™.
Human dental pulp stem cells (hDPs) were isolated and characterized. Extraction medium was prepared from the different pulp capping materials. The hDP cytotoxicity, proliferation, and migration were examined. The odonto/osteogenic differentiation was determined by alkaline phosphatase, Von Kossa, and alizarin red s staining. Osteogenic marker gene expression was evaluated using real-time polymerase chain reaction.
ProRoot® MTA and Biodentine™ generated less cytotoxicity than DyCal® and TheraCal™ LC, which were highly toxic. The hDPs proliferated when cultured with the ProRoot® MTA and Biodentine™ extraction media. The ProRoot® MTA and Biodentine™ extraction medium induced greater cell attachment and spreading. Moreover, the hDPs cultured in the ProRoot® MTA or Biodentine™ extraction medium migrated in a similar manner to those in serum-free medium, while a marked reduction in cell migration was observed in the cells cultured in DyCal® and TheraCal™ LC extraction media. Improved mineralization was detected in hDPs maintained in ProRoot® MTA or Biodentine™ extraction medium compared with those in serum-free medium.
This study demonstrates the favorable in vitro biocompatibility and bioactive properties of ProRoot® MTA and Biodentine™ on hDPs, suggesting their superior regenerative potential compared with DyCal® and TheraCal™.
摘要：
直接盖髓是针尖牙髓暴露的重要牙髓疗法。应用牙髓覆盖材料导致牙本质桥的形成并保护牙髓活力。这项研究的目的是比较四种牙科材料的效果，DyCal®,ProRoot®MTA,Biodentine™,和TheraCal™LC体外。
分离并表征人牙髓干细胞(hDP)。从不同的纸浆封盖材料制备提取介质。hDP的细胞毒性，扩散,和迁移进行了检查。通过碱性磷酸酶测定odto/成骨分化，冯·科萨,和茜素红s染色。使用实时聚合酶链反应评估成骨标记基因表达。
ProRoot®MTA和Biodentine™产生的细胞毒性低于DyCal®和TheraCal™LC，剧毒.当用ProRoot®MTA和Biodentine™提取培养基培养时,hDP增殖。ProRoot®MTA和Biodentine™提取培养基诱导更大的细胞附着和扩散。此外，在ProRoot®MTA或Biodentine™提取培养基中培养的hDP以与无血清培养基中相似的方式迁移，而在DyCal®和TheraCal™LC提取培养基中培养的细胞中观察到细胞迁移的显著减少。与无血清培养基中的那些相比,在ProRoot®MTA或Biodentine™提取培养基中维持的hDP中检测到改善的矿化。
本研究证明了ProRoot®MTA和Biodentine™对hDP具有良好的体外生物相容性和生物活性，表明它们与DyCal®和TheraCal™相比具有优越的再生潜力。
分离并表征人牙髓干细胞(hDP)。从不同的纸浆封盖材料制备提取介质。hDP的细胞毒性，扩散,和迁移进行了检查。通过碱性磷酸酶测定odto/成骨分化，冯·科萨,和茜素红s染色。使用实时聚合酶链反应评估成骨标记基因表达。
ProRoot®MTA和Biodentine™产生的细胞毒性低于DyCal®和TheraCal™LC，剧毒.当用ProRoot®MTA和Biodentine™提取培养基培养时,hDP增殖。ProRoot®MTA和Biodentine™提取培养基诱导更大的细胞附着和扩散。此外，在ProRoot®MTA或Biodentine™提取培养基中培养的hDP以与无血清培养基中相似的方式迁移，而在DyCal®和TheraCal™LC提取培养基中培养的细胞中观察到细胞迁移的显著减少。与无血清培养基中的那些相比,在ProRoot®MTA或Biodentine™提取培养基中维持的hDP中检测到改善的矿化。
本研究证明了ProRoot®MTA和Biodentine™对hDP具有良好的体外生物相容性和生物活性，表明它们与DyCal®和TheraCal™相比具有优越的再生潜力。
