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Abstract：
Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome-wide association and linkage analyses. Fine mapping results showed that soybean resistance to SMV strains G2 and G3 was controlled by a single dominant gene, GmST1, on chromosome 13, encoding a sulfotransferase (SOT). A key variation at position 506 in the coding region of GmST1 associated with the structure of the encoded SOT and changed SOT activity levels between RSVG2-S and RSVG2-R alleles. In RSVG2-S allele carrier \"Hefeng25\", the overexpression of GmST1 carrying the RSVG2-R allele from the SMV-resistant line \"Dongnong93-046\" conferred resistance to SMV strains G2 and G3. Compared to Hefeng25, the accumulation of SMV was decreased in transgenic plants carrying the RSVG2-R allele. SMV infection differentiated both the accumulation of jasmonates and expression patterns of genes involved in jasmonic acid (JA) signalling, biosynthesis and catabolism in RSVG2-R and RSVG2-S allele carriers. This characterization of GmST1 suggests a new scenario explaining soybean resistance to SMV.
摘要：
大豆花叶病毒（SMV）是世界上最广泛和最具破坏性的病毒性疾病之一。大豆对SMV的定性抗性的遗传结构尚不清楚。这里,通过全基因组关联和连锁分析，Rsvg2基因座被鉴定为大豆对SMV的潜在抗性。精细定位结果表明，大豆对SMV菌株G2和G3的抗性受单个显性基因控制，GmST1,在染色体13上,编码磺基转移酶(SOT)。GmST1编码区506位的关键变异与编码SOT的结构相关,并改变了RSVG2-S和RSVG2-R等位基因之间的SOT活性水平。在RSVG2-S等位基因携带者“Hefeng25”中，携带来自SMV抗性系“东农93-046”的RSVG2-R等位基因的GmST1的过表达赋予了对SMV菌株G2和G3的抗性。与Hefeng25相比，携带RSVG2-R等位基因的转基因植物中SMV的积累减少。SMV感染区分了茉莉酸的积累和茉莉酸（JA）信号传导中涉及的基因的表达模式，RSVG2-R和RSVG2-S等位基因携带者的生物合成和分解代谢。GmST1的这种表征表明了解释大豆对SMV抗性的新情况。
