Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review.

全基因组测序时代之前巴西结核分枝杆菌的分子流行病学 ： 文献综述。

• 影响指数: 2.747
• 发表时间: 2021
• 来源期刊: Mem Inst Oswaldo Cruz
• DOI: 10.1590/0074-02760200517
• PMID: 33729319
• 文章类型: Journal Article
• 中科院分区: Q2
• 方向: 医学
• 作者列表: Conceição EC,Salvato RS,Gomes KM,Guimarães AEDS,da Conceição ML,Souza E Guimarães RJP,Sharma A,Furlaneto IP,Barcellos RB,Bollela VR,Anselmo LMP,Sisco MC,Niero CV,Ferrazoli L,Refrégier G,Lourenço MCDS,Gomes HM,de Brito AC,Catanho M,Duarte RS,Suffys PN,Lima KVB

Mesh: Bacterial Typing Techniques; Brazil / epidemiology; Genotype; Humans; Minisatellite Repeats / genetics; Molecular Epidemiology; Mycobacterium tuberculosis / genetics isolation & purification; Polymorphism, Restriction Fragment Length / genetics; Whole Genome Sequencing

Abstract

Molecular-typing can help in unraveling epidemiological scenarios and improvement for disease control strategies. A literature review of Mycobacterium tuberculosis transmission in Brazil through genotyping on 56 studies published from 1996-2019 was performed. The clustering rate for mycobacterial interspersed repetitive units - variable tandem repeats (MIRU-VNTR) of 1,613 isolates were: 73%, 33% and 28% based on 12, 15 and 24-loci, respectively; while for RFLP-IS6110 were: 84% among prison population in Rio de Janeiro, 69% among multidrug-resistant isolates in Rio Grande do Sul, and 56.2% in general population in São Paulo. These findings could improve tuberculosis (TB) surveillance and set up a solid basis to build a database of Mycobacterium genomes.

摘要

分子分型可以帮助揭示流行病学情景并改善疾病控制策略。通过对1996-2019年发表的56项研究进行基因分型，对巴西的结核分枝杆菌传播进行了文献综述。1,613株分枝杆菌散布的重复单元-可变串联重复序列（MIRU-VNTR）的聚集率为：73％，基于12、15和24个基因座的33%和28%，分别；而对于RFLP-IS6110是：里约热内卢监狱人口中的84%，南里奥格兰德州的多药耐药菌株中有69%，在圣保罗的一般人口中占56.2%。这些发现可以改善结核病（TB）监测，并为建立分枝杆菌基因组数据库奠定坚实的基础。