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Abstract：
Male sexual debility affects patients\' confidence and damages the relationship between the couples and thus affects the stability of the family. This study aimed to investigate the effects of oligopeptides isolated from ginseng and oyster (GOPs and OOPs), separately and in combination, on sexual function in male mice. In the first experiment, male mice were randomly divided into five groups: vehicle control group; whey protein (125.0 mg kg-1) group; and GOPs 62.5, 125.0, and 250.0 mg kg-1 groups. In the second experiment, male mice were randomly divided into five groups: vehicle control group, whey protein (160.0 mg kg-1) group, and OOPs 80.0, 160.0, and 320.0 mg kg-1 groups. In the third experiment, male mice were randomly divided into six groups: vehicle control group, whey protein (222.5 mg kg-1) group, and GOPs + OOPs 62.5 + 160.0, 62.5 + 320.0, and 125.0 + 160.0, 125.0 + 320.0 mg kg-1 groups. Test substances were given by gavage once a day for 30 days. The sexual behavior parameters, serum nitric oxide (NO), testosterone, cyclic guanosine monophosphate (cGMP), and phosphodiesterase-5 (PDE5) concentrations were detected. We found that GOPs at 250.0 mg kg-1 improved male sexual behavior, NO, and testosterone content, whereas GOPs at 62.5 and 125.0 mg kg-1 and OOPs at 80.0, 160, and 320 mg kg-1 did not have significant effects. The combination of 62.5 mg kg-1 GOPs + 160.0 mg kg-1 OOPs and the combination of 125.0 mg kg-1 GOPs + 320.0 mg kg-1 OOPs improved male sexual behavior, serum NO, testosterone, and cGMP contents and decreased PDE5 content. The combination of 62.5 mg kg-1 GOPs and 160.0 mg kg-1 OOPs had the best effects among four combined groups. These results suggested that GOPs in combination with OOPs had the synergistic effects of enhancing male sexual function, probably via elevating serum testosterone, NO, and corpus cavernosum cGMP level and decreasing the corpus cavernosum PDE5 level. GOPs and OOPs could be novel natural agents for improving male sexual function.
摘要：
男性性虚弱会影响患者的自信心，损害夫妻之间的关系，从而影响家庭的稳定。本研究旨在研究从人参和牡蛎（GOPs和OOP）中分离的寡肽的作用，单独和组合，雄性小鼠的性功能。在第一个实验中,将雄性小鼠随机分为5组：载体对照组；乳清蛋白（125.0mgkg-1）组；GOPs62.5、125.0和250.0mgkg-1组。在第二个实验中,雄性小鼠随机分为5组:载体对照组,乳清蛋白（160.0mgkg-1）组，和OOPs80.0、160.0和320.0mgkg-1组。在第三个实验中，雄性小鼠随机分为6组:载体对照组,乳清蛋白（222.5mgkg-1）组，和GOPs+OOPs62.5+160.0、62.5+320.0和125.0+160.0、125.0+320.0mgkg-1组。通过每天一次管饲法给予测试物质,持续30天。性行为参数，血清一氧化氮（NO），睾丸激素，环磷酸鸟苷（cGMP），并检测磷酸二酯酶-5(PDE5)浓度。我们发现，250.0mgkg-1的GOP改善了男性的性行为，NO,和睾酮含量，而62.5和125.0mgkg-1的GOP和80.0、160和320mgkg-1的OOP没有显著影响。62.5mgkg-1GOP+160.0mgkg-1OOP和125.0mgkg-1GOP+320.0mgkg-1OOP的组合改善了男性的性行为,血清NO,睾丸激素，cGMP含量和PDE5含量降低。62.5mgkg-1GOP和160.0mgkg-1OOP的组合在四个组合组中具有最佳效果。这些结果表明，GOP与OOP联合具有增强男性性功能的协同作用，可能是通过升高血清睾酮,NO,海绵体cGMP水平降低，海绵体PDE5水平降低。GOP和OOP可能是改善男性性功能的新型天然药物。
