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Abstract：
BACKGROUND: With the undergoing world outbreak of Fusarium wilt of bananas, it is essential to unravel all the possible process of dissemination of this disease. The host-pest interactions of the banana weevil with banana plants make this insect an important potential vector. This study, carried out in controlled conditions, explores the interaction between the banana weevil and Fusarium oxysporum f.sp. cubense race 1 (Foc), with a focus on the external and internal transport of viable fungal propagules.
RESULTS: Viable inoculum of Foc was detected very rapidly on external teguments and in the digestive tract of the insect, i.e. at the lowest time studied of 5 min after contact with infected pseudostems. Maximal inoculum acquisition occurred after 1 h contact with an inoculum source. External inoculum was higher than the inoculum present in the digestive tract, but external and internal inoculum had the same dynamics. After a contact of an infected source, external and internal inoculum decreased exponentially within 50 h, but weevils remained infested for a long time, as long as 2 or 3 days that would be enough for inoculum dispersal. Viable inoculum was also detected in feces. Foc strains isolated were pathogenic when inoculated to banana plants of the Gros Michel variety but did not provoke any symptom on Cavendish banana plants.
CONCLUSIONS: These results demonstrate that the infective structures of Foc remain externally viable in the digestive system and the excreta of the banana weevil. Such excreta are capable of making healthy banana plants of the Gros Michel variety. © 2021 Society of Chemical Industry.
RESULTS: Viable inoculum of Foc was detected very rapidly on external teguments and in the digestive tract of the insect, i.e. at the lowest time studied of 5 min after contact with infected pseudostems. Maximal inoculum acquisition occurred after 1 h contact with an inoculum source. External inoculum was higher than the inoculum present in the digestive tract, but external and internal inoculum had the same dynamics. After a contact of an infected source, external and internal inoculum decreased exponentially within 50 h, but weevils remained infested for a long time, as long as 2 or 3 days that would be enough for inoculum dispersal. Viable inoculum was also detected in feces. Foc strains isolated were pathogenic when inoculated to banana plants of the Gros Michel variety but did not provoke any symptom on Cavendish banana plants.
CONCLUSIONS: These results demonstrate that the infective structures of Foc remain externally viable in the digestive system and the excreta of the banana weevil. Such excreta are capable of making healthy banana plants of the Gros Michel variety. © 2021 Society of Chemical Industry.
摘要：
背景：随着世界香蕉枯萎病的爆发，必须解开这种疾病传播的所有可能过程。香蕉象鼻虫与香蕉植物的寄主-害虫相互作用使这种昆虫成为重要的潜在媒介。这项研究,在受控条件下进行，探讨了香蕉象鼻虫和尖孢镰刀菌之间的相互作用。cubense种族1（Foc），专注于活真菌繁殖体的外部和内部运输。
结果：在昆虫的外部盖和消化道中非常迅速地检测到Foc的活菌接种物，即在与受感染的假茎接触后5分钟研究的最低时间。与接种物源接触1小时后发生最大接种物采集。外部接种物高于消化道中存在的接种物，但是外部和内部接种物具有相同的动力学。在接触感染源之后，外部和内部接种物在50小时内呈指数下降，但是象鼻虫感染了很长时间，只要2或3天，这将是足够的接种物扩散。在粪便中也检测到有活力的接种物。将分离出的Foc菌株接种到GrosMichel品种的香蕉植物上时具有致病性，但在卡文迪许香蕉植物上没有引起任何症状。
结论：这些结果表明，Foc的感染结构在消化系统和香蕉象鼻虫的排泄物中保持外部活力。这种排泄物能够制造GrosMichel品种的健康香蕉植物。©2021年化学工业学会。
结果：在昆虫的外部盖和消化道中非常迅速地检测到Foc的活菌接种物，即在与受感染的假茎接触后5分钟研究的最低时间。与接种物源接触1小时后发生最大接种物采集。外部接种物高于消化道中存在的接种物，但是外部和内部接种物具有相同的动力学。在接触感染源之后，外部和内部接种物在50小时内呈指数下降，但是象鼻虫感染了很长时间，只要2或3天，这将是足够的接种物扩散。在粪便中也检测到有活力的接种物。将分离出的Foc菌株接种到GrosMichel品种的香蕉植物上时具有致病性，但在卡文迪许香蕉植物上没有引起任何症状。
结论：这些结果表明，Foc的感染结构在消化系统和香蕉象鼻虫的排泄物中保持外部活力。这种排泄物能够制造GrosMichel品种的健康香蕉植物。©2021年化学工业学会。
