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Abstract：
Digital dermatitis (DD) lesions in cattle are characterized by the presence of multiple Treponema species. Current culture media for isolating treponemes generally uses serum supplementation from different animals to target particular Treponema sp.; however, their suitability for DD Treponema isolation has not been fully determined. We studied the effect of culture media (OTEB, NOS and TYGV) and serum supplementation on mixed Treponema spp. dynamics. Bacterial growth was evaluated by direct microscopic count, optical density, wet weight and a species-specific qPCR and the correlations between these independent methods were calculated. Wet weight, optical density and bacterial count correlated best with each other. Different Treponema species performed differently under the tested culture media. T. phagedenis growth was enhanced in OTEB media supplemented with bovine fetal serum (BFS) or horse serum (HS). T. medium had lower generation time when culture media were supplemented with rabbit serum (RS). Lowest generation time for T. pedis and T. denticola were obtained in NOS media supplemented with HS and OTEB media supplemented with BFS, respectively. Detection of cystic forms observed after 5 days of culture did not differ among the culture media. Correlation between different Treponema spp. growth quantification techniques indicated that alternative quantification methods such as qPCR and wet weight could be used depending on the purpose. We conclude that effects of culture media and serum supplementation on mixed Treponema spp. communities should be taken into account when isolating a specific Treponema species.
摘要：
牛的数字皮炎（DD）病变的特征是存在多种密螺旋体。目前用于分离密螺旋体的培养基通常使用来自不同动物的血清补充剂来靶向特定的密螺旋体。;然而,他们是否适合DD密螺旋体分离尚未完全确定.我们研究了培养基的影响(OTEB，NOS和TYGV）和混合密螺旋体的血清补充。动态。通过直接显微镜计数评估细菌生长，光密度,计算湿重和物种特异性qPCR以及这些独立方法之间的相关性。湿重,光密度和细菌计数之间的相关性最好。不同的密螺旋体物种在测试的培养基下表现不同。在补充有牛胎儿血清(BFS)或马血清(HS)的OTEB培养基中,增强了噬菌丝生长。当培养基补充兔血清(RS)时,T.培养基具有较低的生成时间。在补充有HS的NOS培养基和补充有BFS的OTEB培养基中获得T.pedis和T.denticola的最低生成时间，分别。培养5天后观察到的囊性形式的检测在培养基之间没有差异。不同螺旋体属的相关性.生长定量技术表明，根据目的，可以使用其他定量方法，例如qPCR和湿重。我们得出的结论是，培养基和血清补充对混合密螺旋体的影响。在分离特定密螺旋体物种时，应考虑社区。
