Abstract：
Coronary artery disease is an inflammatory disease. Systemic markers of inflammation such as Interleukin-6, Tumor Necrosis Factor alpha and C-reactive protein have previously been shown to be associated with increased risk of cardiovascular events. The aim of the present study is to assess the role of variants in the IL-6 (- 174 G/C), TNFα (- 308 A/G) and CRP (+ 1059G/C) genes as susceptibility markers for CAD in a Tunisian population. The investigation was conducted as a case-control study involving 204 patients and 400 age-gender matched controls. Genotyping was performed using polymerase chain reaction and restriction fragment length polymorphism analysis. There are significant differences between CAD patients and the control group with regard to BMI (p < 10-3) and family history of CAD (p < 10-3). The CAD patients are more likely to have a history of smoking (p < 10-3), have a higher value of TC (p = 0.003), LDLc (p = 0.016), hs-CRP (p = 0.01), IL6 (p < 10-3) and TNFα (p = 0.038). Our analysis showed significant differences between cases and controls in genotypic distribution of IL6-174CC (p = 0.003; OR = 7.71 CI (1.58-37.56)), TNFα - 308 AA (p = 0.004; OR = 2.95 (1.57-5.51)) and CRP + 1059 CC (p < 10-3; OR = 5.40 (2.30-12.68)). However, we failed to find an association between the different genotypes and the inflammatory markers levels. Our results suggest that the presence of IL-6 (- 174 G/C), TNFα (-308 A/G) and CRP (+ 1059G/C) polymorphisms, may be considered to be a risk factor for CAD in Tunisian population.
摘要：
冠状动脉疾病是一种炎性疾病。炎症的系统性标志物，例如白细胞介素-6，肿瘤坏死因子α和C反应蛋白先前已被证明与心血管事件的风险增加有关。本研究的目的是评估变体在IL-6（-174G/C）中的作用，TNFα(-308A/G)和CRP(+1059G/C)基因作为突尼斯人群中CAD的易感性标记。这项调查是作为病例对照研究进行的，涉及204名患者和400名年龄性别匹配的对照。使用聚合酶链反应和限制性片段长度多态性分析进行基因分型。在BMI（p<10-3）和CAD家族史（p<10-3）方面，CAD患者与对照组之间存在显着差异。CAD患者更有可能有吸烟史（p<10-3），具有较高的TC值(p=0.003)，LDLc（p=0.016），hs-CRP（p=0.01），IL6(p<10-3)和TNFα(p=0.038)。我们的分析显示，病例和对照在IL6-174CC的基因型分布方面存在显着差异（p=0.003;OR=7.71CI（1.58-37.56）），TNFα-308AA(p=0.004;OR=2.95(1.57-5.51))和CRP+1059CC(p<10-3;OR=5.40(2.30-12.68))。然而,我们未能发现不同基因型与炎症标志物水平之间的关联.我们的结果表明，IL-6的存在（-174G/C），TNFα（-308A/G）和CRP（+1059G/C）多态性，可能被认为是突尼斯人群CAD的危险因素。
