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Abstract：
The nuclear pore complex (NPC) is the proteinaceous nanopore that solely mediates the transport of both small molecules and macromolecules between the nucleus and cytoplasm of a eukaryotic cell to regulate gene expression. In this personal account, we introduce recent progress in our nanoelectrochemical study of molecular transport through the NPC. Our work represents the importance of chemistry in understanding and controlling of NPC-mediated molecular transport to enable the efficient and safe delivery of genetic therapeutics into the nucleus, thereby fundamentally contributing to human health. Specifically, we employ nanoscale scanning electrochemical microscopy to test our hypothesis that the nanopore of the NPC is divided by transport barriers concentrically into peripheral and central routes to efficiently mediate the bimodal traffic of protein transport and RNA export, respectively, through cooperative hydrophobic and electrostatic interactions.
摘要：
核孔复合物(NPC)是蛋白质纳米孔,其仅介导小分子和大分子在真核细胞的细胞核和细胞质之间的转运以调节基因表达。在这个个人账户中，我们介绍了通过NPC的分子传输的纳米电化学研究的最新进展。我们的工作代表了化学在理解和控制NPC介导的分子转运方面的重要性，以使基因疗法能够有效和安全地输送到细胞核。从根本上促进人类健康。具体来说,我们采用纳米级扫描电化学显微镜来检验我们的假设，即NPC的纳米孔被运输屏障同心地分为外周和中心路线，以有效地介导蛋白质运输和RNA输出的双峰运输。分别,通过协同疏水和静电相互作用。
