PDF(Pubmed)
Abstract：
UNASSIGNED: Natural topical products have been used to enhance wound healing, especially in immunocompromised animals. The aims of this study were to evaluate and to compare the effects of moist exposed burn ointment (MEBO) and honey on the healing of full-thickness skin wounds in immunocompromised dogs.
UNASSIGNED: The study was conducted using 12 adults, apparently healthy mongrel dogs. Immunosuppression was induced in six dogs by oral administration of prednisone (2 mg/kg) and azathioprine (2 mg/kg), once a day for 21 days. On each dog, a total of 9, 1.5 cm in diameter full-thickness skin circular wounds were created aseptically in the thoracolumbar area under general anesthesia using customized skin punch biopsy kit. In a random fashion, three wounds in each dog were treatment using MEBO (contains b-sitosterol, baicalin, and berberine as active ingredients in a base of beeswax and sesame oil), honey or no treatment (control), once per day for 21 days. Wounds were grossly evaluated once a day for signs of inflammation or infection. In addition, biopsy specimens and digital imaging data of each wound were obtained on days 7, 14, and 21 for histopathological evaluation of the healing process.
UNASSIGNED: Wounds in immunocompromised dogs appeared to heal significantly in a slower fashion than in non-immunocompromised counterparts. Digital analysis data showed that MEBO-treated wounds expressed better epithelialization area, faster contraction, and smaller wound area percentage when compared with honey-treated wounds. Histopathological analysis showed significantly higher angiogenesis scores in MEBO-treated wounds when compared with other treatments.
UNASSIGNED: Results of this study showed that MEBO resulted in significant enhancement of wound healing in both healthy and immunocompromised dogs. However, when compared to honey, the wound healing effect of MEBO was superior to that of honey.
UNASSIGNED: The study was conducted using 12 adults, apparently healthy mongrel dogs. Immunosuppression was induced in six dogs by oral administration of prednisone (2 mg/kg) and azathioprine (2 mg/kg), once a day for 21 days. On each dog, a total of 9, 1.5 cm in diameter full-thickness skin circular wounds were created aseptically in the thoracolumbar area under general anesthesia using customized skin punch biopsy kit. In a random fashion, three wounds in each dog were treatment using MEBO (contains b-sitosterol, baicalin, and berberine as active ingredients in a base of beeswax and sesame oil), honey or no treatment (control), once per day for 21 days. Wounds were grossly evaluated once a day for signs of inflammation or infection. In addition, biopsy specimens and digital imaging data of each wound were obtained on days 7, 14, and 21 for histopathological evaluation of the healing process.
UNASSIGNED: Wounds in immunocompromised dogs appeared to heal significantly in a slower fashion than in non-immunocompromised counterparts. Digital analysis data showed that MEBO-treated wounds expressed better epithelialization area, faster contraction, and smaller wound area percentage when compared with honey-treated wounds. Histopathological analysis showed significantly higher angiogenesis scores in MEBO-treated wounds when compared with other treatments.
UNASSIGNED: Results of this study showed that MEBO resulted in significant enhancement of wound healing in both healthy and immunocompromised dogs. However, when compared to honey, the wound healing effect of MEBO was superior to that of honey.
摘要：
■天然外用产品已用于增强伤口愈合，尤其是在免疫功能低下的动物中。这项研究的目的是评估和比较湿润烧伤软膏（MEBO）和蜂蜜对免疫功能低下的狗的全层皮肤伤口愈合的影响。
■这项研究是使用12名成年人进行的，显然健康的杂种狗。通过口服泼尼松（2mg/kg）和硫唑嘌呤（2mg/kg）在6只狗中诱导免疫抑制，每天一次，共21天。每只狗,在全身麻醉下,使用定制的皮肤穿孔活检试剂盒,在胸腰椎区无菌创建了总共9,1.5cm直径的全层皮肤圆形伤口.以一种随机的方式，使用MEBO治疗每只狗的三个伤口（含有b-谷甾醇，黄芩苷,和黄连素作为蜂蜡和芝麻油基质中的活性成分），蜂蜜或不治疗（对照），每天一次，共21天。每天一次严重评估伤口的炎症或感染迹象。此外,在第7,14和21天获得每个伤口的活检标本和数字成像数据,用于愈合过程的组织病理学评估.
■免疫受损的狗的伤口似乎比非免疫受损的狗以缓慢的方式愈合。数字分析数据显示，MEBO处理的伤口表现出更好的上皮形成面积，更快的收缩，与蜂蜜治疗的伤口相比，伤口面积百分比较小。组织病理学分析显示，与其他治疗相比，MEBO治疗的伤口的血管生成得分明显更高。
■本研究的结果表明,MEBO导致健康和免疫受损的狗的伤口愈合的显著增强。然而,与蜂蜜相比，湿润烧伤膏的创面愈合效果优于蜂蜜。
■这项研究是使用12名成年人进行的，显然健康的杂种狗。通过口服泼尼松（2mg/kg）和硫唑嘌呤（2mg/kg）在6只狗中诱导免疫抑制，每天一次，共21天。每只狗,在全身麻醉下,使用定制的皮肤穿孔活检试剂盒,在胸腰椎区无菌创建了总共9,1.5cm直径的全层皮肤圆形伤口.以一种随机的方式，使用MEBO治疗每只狗的三个伤口（含有b-谷甾醇，黄芩苷,和黄连素作为蜂蜡和芝麻油基质中的活性成分），蜂蜜或不治疗（对照），每天一次，共21天。每天一次严重评估伤口的炎症或感染迹象。此外,在第7,14和21天获得每个伤口的活检标本和数字成像数据,用于愈合过程的组织病理学评估.
■免疫受损的狗的伤口似乎比非免疫受损的狗以缓慢的方式愈合。数字分析数据显示，MEBO处理的伤口表现出更好的上皮形成面积，更快的收缩，与蜂蜜治疗的伤口相比，伤口面积百分比较小。组织病理学分析显示，与其他治疗相比，MEBO治疗的伤口的血管生成得分明显更高。
■本研究的结果表明,MEBO导致健康和免疫受损的狗的伤口愈合的显著增强。然而,与蜂蜜相比，湿润烧伤膏的创面愈合效果优于蜂蜜。
