Abstract：
Neutrophil migration to the site of infection is an essential process for the control and clearance of microbial growth within the host. Identifying the molecular factors that mediate neutrophil chemotaxis is therefore critical for our understanding of disease pathogenesis and the mechanisms underlying protective immunity. Here, we describe a protocol that enables analysis of neutrophil recruitment from the blood into fungal-infected organs in vivo, using mixed bone marrow chimeras and flow cytometry. This method directly assesses the relative contribution of a receptor or intracellular molecule in controlling neutrophil chemotaxis during fungal infection and can be adapted to a variety of other non-fungal infection experimental settings.
摘要：
中性粒细胞迁移到感染部位是控制和清除宿主内微生物生长的重要过程。因此，鉴定介导中性粒细胞趋化性的分子因素对于我们理解疾病的发病机理和保护性免疫机制至关重要。这里,我们描述了一个协议，使中性粒细胞募集从血液到体内真菌感染器官的分析，使用混合骨髓嵌合体和流式细胞术。该方法直接评估受体或胞内分子在真菌感染期间控制嗜中性粒细胞趋化性中的相对贡献，并且可以适应各种其他非真菌感染实验设置。
