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Abstract：
Entomopathogenic bacteria and fungi are quite frequently found in soils and insect cadavers. The first step in utilizing these microbes as biopesticides is to isolate them, and several culture media and insect baiting procedures have been tested in this direction. In this work, the authors review the current techniques that have been developed so far, in the last five decades, and display brief protocols which can be adopted for the isolations of these entomopathogens. Among bacteria, this review focuses on Serratia spp. and bacteria from the class Bacilli. Among fungi, the review focuses those from the order Hypocreales, for example, genera Beauveria, Clonostachys, Lecanicillium, Metarhizium, and Purpureocillium. The authors chose these groups of entomopathogenic bacteria and fungi based on their importance in the microbial biopesticide market.
摘要：
在土壤和昆虫尸体中经常发现昆虫病原细菌和真菌。利用这些微生物作为生物农药的第一步是分离它们，在这个方向上已经测试了几种培养基和昆虫诱饵程序。在这项工作中,作者回顾了到目前为止已经开发的当前技术，在过去的五十年里，并显示可用于分离这些昆虫病原体的简短协议。在细菌中，这篇综述的重点是沙雷氏菌。和芽孢杆菌类的细菌。在真菌中,这篇评论的重点是来自Hypecreales订单的那些，例如,白僵菌属,Clonostachys,Lecanicillium,绿僵菌，和Purpureocillium.作者根据它们在微生物生物农药市场中的重要性选择了这些昆虫病原细菌和真菌。
