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Abstract：
Demand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid-liquid extraction methods requiring large volumes of various organic solvents and time. To address this question from a different angle, we proposed, here, to investigate the use of three different aqueous two-phase extraction systems using either ammonium- or imidazolium-based ionic liquids. We applied these to four fermentation broths of Talaromyces albobiverticillius (deep red pigment producer), Emericella purpurea (red pigment producer), Paecilomyces marquandii (yellow pigment producer) and Trichoderma harzianum (yellow-brown pigment producer) to investigate their selective extraction abilities towards the detection of polyketide-based pigments. Our findings led us to conclude that (i) these alternative extraction systems using ionic liquids as greener extractant means worked well for this extraction of colored molecules from the fermentation broths of the filamentous fungi investigated here; (ii) tetrabutylammonium bromide, [N4444]Br-, showed the best pigment extraction ability, with a higher putative affinity for azaphilone red pigments; (iii) the back extraction and recovery of the fungal pigments from ionic liquid phases remained the limiting point of the method under our selected conditions for potential industrial applications. Nevertheless, these alternative extraction procedures appeared to be promising ways for the detection of polyketide-based colorants in the submerged cultures of filamentous fungi.
摘要：
对微生物着色剂的需求现在正成为食品的竞争性研究课题，化妆品和制药业。在大多数应用中,通过需要大量的各种有机溶剂和时间的常规液-液提取方法来提取感兴趣的色素,例如来自真菌浸没培养物的基于聚酮化合物的红色色素。为了从不同的角度解决这个问题，我们提议,在这里，研究使用基于氨或咪唑的离子液体的三种不同的双水相萃取系统。我们将这些应用于四个albobiverticillius（深红色色素生产商）的发酵液，紫癜（红色素生产者），Marquandii拟青霉（黄色素生产者）和哈茨木霉（黄褐色色素生产者）研究了它们对检测聚酮基色素的选择性提取能力。我们的发现使我们得出结论：（i）这些使用离子液体作为绿色萃取剂的替代萃取系统对于从此处研究的丝状真菌的发酵液中提取有色分子效果良好；（ii）溴化四丁基铵，[N4444]Br-,表现出最佳的色素提取能力，对氮杂酮红色颜料具有较高的推定亲和力；（iii）在我们为潜在的工业应用选择的条件下，从离子液体相中反萃取和回收真菌颜料仍然是该方法的限制点。然而，这些替代的提取程序似乎是在丝状真菌的浸没培养物中检测基于聚酮化合物的着色剂的有希望的方法。
