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Abstract：
Previous studies have reported that phospholipids rich in n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA) in the form of liposome exhibited superior bioactivities than other formulation. However, the digestion and absorption characteristics of n-3 LCPUFA-enriched phospholipids were still unclear, restricting the molecular mechanism analysis related to their distinctive activities. The aim of the present study was to compare the digestion and absorption characteristics of DHA/EPA-PC in the forms of liposome and emulsion. The fatty acid composition and lipid species in serum, intestinal wall and content of healthy mice were determined after oral administration with DHA/EPA-PC. Results showed that the peak value of serum DHA/EPA level in the liposome group was significantly higher than that of the emulsion group (p < 0.05), although the peak in the liposome group appeared at 3 h and the peak time was 2 h in the emulsion group. Lipidomics analysis indicated that the high levels of total PL and PL-DHA could be retained in serum for a substantial period after administration of the DHA/EPA-PC liposome, which might be attributed to that the DHA/EPA-PC in the form of liposomes was hydrolyzed slower by pancreatic phospholipase A2 than the emulsion form in small intestinal content. The obtained results might provide theoretical basis for the development and utilization of marine-derived phospholipids.
摘要：
先前的研究已经报道,脂质体形式的富含n-3长链多不饱和脂肪酸(n-3LCPUFA)的磷脂表现出比其他制剂优异的生物活性。然而,富含n-3LCPUFA的磷脂的消化吸收特性尚不清楚,限制了与其独特活性相关的分子机制分析。本研究的目的是比较脂质体和乳剂形式的DHA/EPA-PC的消化和吸收特性。血清中的脂肪酸组成和脂质种类，口服DHA/EPA-PC后测定健康小鼠的肠壁和含量。结果显示,脂质体组血清DHA/EPA水平峰值明显高于乳剂组(p<0.05),脂质体组峰出现在3h，乳剂组峰时间为2h。脂质组学分析表明，服用DHA/EPA-PC脂质体后，血清中高水平的总PL和PL-DHA可以保留相当长的时间。这可能归因于脂质体形式的DHA/EPA-PC被胰腺磷脂酶A2水解的速度比小肠内容物中的乳液形式慢。所得结果可为海洋磷脂的开发利用提供理论依据。
