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Abstract：
The use of matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI) directly on urine can significantly improve turnaround time for diagnosing urinary tract infection (UTI). The present study was planned to compare the performance of MALDI directly on urine samples with conventional urine culture and evaluate an algorithm using a combination of significant pyuria and MALDI directly on urine samples as a screening method for diagnosing UTI.
A total of 1000 urine samples from patients with symptoms suggestive of UTIs were analysed. Urine microscopy, urine culture and MALDI were performed simultaneously on all the samples.
MALDI correctly identified the microorganisms in 73.83% monomicrobial samples. In culture showing a mixed growth of two and more than three organisms, MALDI was able to identify one microorganism in 27.58% and 15.78% of samples, respectively. There were no peaks by MALDI in 93.53% of 464 sterile samples. The sequential algorithm using urine microscopy and MALDI could correctly identify UTI in 66.23% cases.
MALDI can be utilised to rule out bacteriuria in >93% of sterile urine samples. The combination of significant pyuria and direct MALDI as screening method to diagnose UTI (whether monomicrobial or polymicrobial) was not found to be superior than using direct MALDI on urine samples alone.
A total of 1000 urine samples from patients with symptoms suggestive of UTIs were analysed. Urine microscopy, urine culture and MALDI were performed simultaneously on all the samples.
MALDI correctly identified the microorganisms in 73.83% monomicrobial samples. In culture showing a mixed growth of two and more than three organisms, MALDI was able to identify one microorganism in 27.58% and 15.78% of samples, respectively. There were no peaks by MALDI in 93.53% of 464 sterile samples. The sequential algorithm using urine microscopy and MALDI could correctly identify UTI in 66.23% cases.
MALDI can be utilised to rule out bacteriuria in >93% of sterile urine samples. The combination of significant pyuria and direct MALDI as screening method to diagnose UTI (whether monomicrobial or polymicrobial) was not found to be superior than using direct MALDI on urine samples alone.
摘要：
直接在尿液上使用基质辅助激光解吸/电离飞行时间质谱（MALDI）可以显着改善诊断尿路感染（UTI）的周转时间。本研究计划将MALDI直接在尿液样本上的性能与常规尿液培养进行比较，并评估使用明显的脓尿和MALDI直接在尿液样本上的组合作为诊断UTI的筛查方法的算法。
分析了来自症状提示UTI的患者的总共1000个尿液样本。尿液显微镜检查,对所有样本同时进行尿培养和MALDI.
MALDI正确鉴定了73.83%的单抗微生物样品中的微生物。在显示两种和三种以上生物混合生长的培养物中，MALDI能够在27.58%和15.78%的样本中鉴定出一种微生物，分别。在464个无菌样品的93.53%中,MALDI没有峰。使用尿液显微镜和MALDI的顺序算法可以正确识别66.23％的UTI。
MALDI可用于排除>93％的无菌尿液样品中的细菌尿。未发现显著脓尿和直接MALDI作为诊断UTI(无论是单微生物还是多微生物)的筛选方法的组合优于单独对尿液样品使用直接MALDI。
分析了来自症状提示UTI的患者的总共1000个尿液样本。尿液显微镜检查,对所有样本同时进行尿培养和MALDI.
MALDI正确鉴定了73.83%的单抗微生物样品中的微生物。在显示两种和三种以上生物混合生长的培养物中，MALDI能够在27.58%和15.78%的样本中鉴定出一种微生物，分别。在464个无菌样品的93.53%中,MALDI没有峰。使用尿液显微镜和MALDI的顺序算法可以正确识别66.23％的UTI。
MALDI可用于排除>93％的无菌尿液样品中的细菌尿。未发现显著脓尿和直接MALDI作为诊断UTI(无论是单微生物还是多微生物)的筛选方法的组合优于单独对尿液样品使用直接MALDI。
