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Abstract：
The study aims to perform, for the first time, the molecular identification of anisakid larvae in commercial fish from the Southeastern Pacific Ocean off the Peru coast, and to provide data on their infection level by fishing ground, fish host, and site of infection. Fish specimens (N = 348) from the northern and the central coast of Peru were examined for parasites. The fish fillets were examined by the UV-press method. Anisakis spp. larvae (N = 305) were identified by mtDNA cox2 sequences analysis and by the ARMS-PCR of the locus nas10 nDNA. Two hundred and eighty-eight Anisakis Type I larvae corresponded to Anisakis pegreffii, whereas 17 Anisakis Type II larvae clustered in a phylogenetic lineage distinct from Anisakis physeteris deposited in GenBank, and corresponding to a phylogenetic lineage indicated as Anisakis sp. 2, previously detected in fish from both Pacific and Atlantic waters. Anisakis pegreffii was found to infect both the flesh and viscera, while Anisakis sp. 2 occurred only in the viscera. The average parasitic burden with A. pegreffii in the examined fish species from the two fishing grounds was significantly higher than that observed with Anisakis sp. 2. The results obtained contribute to improve the knowledge on the distribution and occurrence of Anisakis species in Southeastern Pacific waters and their implications in seafood safety for the local human populations.
摘要：
这项研究旨在执行，第一次,来自秘鲁沿海东南太平洋的商业鱼类的anisakid幼虫的分子鉴定，并提供渔场感染水平的数据，鱼宿主,和感染部位。对秘鲁北部和中部海岸的鱼类标本（N=348）进行了寄生虫检查。通过UV压榨方法检查鱼片。异尖菌种。通过mtDNAcox2序列分析和nas10nDNA基因座的ARMS-PCR鉴定了幼虫（N=305）。与Anisakispegreffii相对应的是288个AnisakisI型幼虫，而17个II型异语幼虫聚集在一个系统发育谱系中，与存放在GenBank中的异语幼虫不同，并对应于表示为Anisakissp的系统发育谱系。2，以前在太平洋和大西洋水域的鱼类中检测到。异见被发现感染了肉和内脏，而Anisakissp.2只发生在内脏。来自两个渔场的所检查鱼类中的A.pegreffii的平均寄生负荷显着高于Anisakissp。2.获得的结果有助于提高对东南太平洋水域Anisakis物种的分布和发生及其对当地人群海鲜安全的影响的认识。
