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Abstract：
OBJECTIVE: Precise pollen collection methods are necessary for crop breeding, but anemophilous pollen is notoriously difficult to capture and control. Here we compared a variety of methods for the controlled capture of cannabis pollen, intended to ease the process of cross-fertilization for breeding this wind-pollinated plant, and measured the utility of light spectroscopy for quantifying relative pollen yield.
RESULTS: In two independent trials, we compared a control method of pollen collection (hand collection) to either vacuum-, water-, or bag-collection methods. We used visible light spectroscopy to quantify relative pollen yield, and validated this approach using microscopic pollen counts. We determined that pollen yield was highest when using hand collection or vacuum collection, but efficiency did not differ significantly among methods.
CONCLUSIONS: To maximize yield, pollen should be collected by hand or vacuum, but all collection methods were equally efficient in a relative sense because yield increased with collection time. We also found that light spectroscopy is an accurate and rapid method of quantifying pollen abundance (R 2 = 0.86) in a liquid suspension.
RESULTS: In two independent trials, we compared a control method of pollen collection (hand collection) to either vacuum-, water-, or bag-collection methods. We used visible light spectroscopy to quantify relative pollen yield, and validated this approach using microscopic pollen counts. We determined that pollen yield was highest when using hand collection or vacuum collection, but efficiency did not differ significantly among methods.
CONCLUSIONS: To maximize yield, pollen should be collected by hand or vacuum, but all collection methods were equally efficient in a relative sense because yield increased with collection time. We also found that light spectroscopy is an accurate and rapid method of quantifying pollen abundance (R 2 = 0.86) in a liquid suspension.
摘要：
目的：精确的花粉采集方法对于作物育种是必要的，但是众所周知，嗜血花粉很难捕获和控制。在这里，我们比较了控制捕获大麻花粉的各种方法，旨在缓解这种风授粉植物的杂交受精过程，并测量了光谱技术用于定量相对花粉产量的实用性。
结果：在两项独立试验中，我们将花粉采集(手采集)的控制方法与真空相比，水-,或收集袋的方法。我们使用可见光光谱法来量化相对花粉产量，并使用微观花粉计数验证了这种方法。我们确定使用手工采集或真空采集时花粉产量最高，但不同方法的效率没有显著差异.
结论：为了最大限度地提高产量，花粉应该用手或真空收集，但是所有收集方法在相对意义上都是同样有效的，因为产量随着收集时间的增加而增加。我们还发现，光谱法是定量液体悬浮液中花粉丰度（R2=0.86）的准确而快速的方法。
结果：在两项独立试验中，我们将花粉采集(手采集)的控制方法与真空相比，水-,或收集袋的方法。我们使用可见光光谱法来量化相对花粉产量，并使用微观花粉计数验证了这种方法。我们确定使用手工采集或真空采集时花粉产量最高，但不同方法的效率没有显著差异.
结论：为了最大限度地提高产量，花粉应该用手或真空收集，但是所有收集方法在相对意义上都是同样有效的，因为产量随着收集时间的增加而增加。我们还发现，光谱法是定量液体悬浮液中花粉丰度（R2=0.86）的准确而快速的方法。
