A case-control study of microRNA polymorphisms in gastric cancer screening by SNP chip combined with time of flight mass spectrometry.

• 影响指数: 2.498
• 发表时间: 11 2020
• 来源期刊: Biomark Med
• DOI: 10.2217/bmm-2020-0003
• PMID: 32927965
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 医学
• 作者列表: Wu C,Han R,Yang S,Jiang Y,Shu Z,Liu J,Ji S,Yan W,Liu B

关键词: SNP; cancer risk; case–control; epidemiology; gastric cancer

Mesh: Aged; Biomarkers, Tumor / genetics; Case-Control Studies; China; Early Detection of Cancer / methods; Female; Genetic Predisposition to Disease / genetics; Humans; Male; Mass Spectrometry / methods; MicroRNAs / analysis genetics metabolism; Middle Aged; Oligonucleotide Array Sequence Analysis; Polymorphism, Single Nucleotide / genetics; Stomach Neoplasms / diagnosis genetics

Abstract

Aims: To explore new SNP sites of miRNAs associated with gastric cancer, thereby providing valuable biomarkers to diagnose and screen gastric cancer. Materials & methods: A 1:1 case-control study was carried out. Microarrays were used to screen the SNP loci of miRNAs in the genomes of matched pairs of patients, 96 with gastric cancer and 96 healthy controls. For validation, mass spectrometry was used to classify miRNA SNP loci in 622 pairs of subjects. Results: rs7143252 was linked to a higher occurrence of gastric cancer. Conclusion: These results suggest that rs7143252 could be used as a specific biomarker to diagnose and screen gastric cancer.