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Abstract：
The objective was to develop a laboratory procedure to validate American Urological Association (AUA) Guideline on vasectomy success when nonmotile spermatozoa are found in the post-vasectomy ejaculate. The neutral α-glucosidase (NAG) an epididymal protein assay modified to determine the activity at 30 and 90 min of incubation from 24 pre- and 47 post-vasectomy ejaculates. The difference between the two points in the relative activity was calculated and if the difference was nonsignificant will confirm vasectomy success. The mean differences in the relative NAG activity were significantly different in pre- and post-vasectomy ejaculates, respectively. The mean differences in the relative NAG activity were similar in post-vasectomy ejaculates with and without nonmotile spermatozoa. No difference in relative NAG activity in post-vasectomy ejaculates between two time points of incubation may be a reliable method to confirm occlusion of the vas deferens. It also validates the recommendation by AUA Guideline on vasectomy success in the presence of few nonmotile spermatozoa.
摘要：
目的是开发一种实验室程序，以验证在输精管切除术后射精中发现非运动精子时，美国泌尿外科协会（AUA）关于输精管切除术成功的指南。中性α-葡萄糖苷酶（NAG）是一种附睾蛋白测定法，经过修改，可以确定24种输精管切除术前和47种输精管切除术后射精在孵育30和90分钟时的活性。计算相对活性的两点之间的差异，如果差异不显著，将确认输精管切除术成功。输精管切除术前和后射精中相对NAG活性的平均差异显着不同，分别。在有和没有不运动精子的输精管切除术后射精中，相对NAG活性的平均差异相似。两个孵育时间点之间输精管切除术后射精的相对NAG活性没有差异可能是确认输精管闭塞的可靠方法。它还验证了AUA指南关于在少数不运动精子存在下输精管切除术成功的建议。
