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Abstract：
Until recently it seemed that amoebae of the genus Thecamoeba can be reliably identified using light-microscopic characters, like the size and shape of the cell, a characteristic pattern of folds and ridges and structure of the nucleus. However, recent molecular studies show the presence of sibling species that can be reliably distinguished based on the gene sequence data only. Here we describe a new terrestrial species, Thecamoeba foliovenanda n. sp. This species is almost identical with Thecamoeba similis in light-microscopy, which has minor differences in the ultrastructure but considerable differences in the SSU rRNA gene sequence. We investigated the light-microscopic data, as well as transmission electron-microscopic images and videorecords on the type strain of T. similis 1583/8 and performed its comparison with the new species. This study further supports the conclusion that species of the genus Thecamoeba nowadays require gene sequencing for reliable identification and species distinction.
摘要：
直到最近，似乎可以使用光学显微镜特征可靠地识别出Tecamoeba属的变形虫，比如细胞的大小和形状，褶皱和脊的特征图案和核的结构。然而,最近的分子研究表明，仅根据基因序列数据可以可靠地区分同胞物种的存在。这里我们描述了一种新的陆地物种，叶阿米巴n.sp.在光学显微镜下，该物种几乎与Thecamoebasimilis相同，超微结构差异较小，但SSUrRNA基因序列差异很大。我们调查了光学显微镜数据，以及T.similis1583/8型菌株的透射电子显微镜图像和录像带，并与新物种进行了比较。这项研究进一步支持了这样的结论，即如今的皮卡米巴属物种需要基因测序才能进行可靠的鉴定和物种区分。
