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Abstract：
Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite candidates selected after NGS analysis, 126 yielded PCR products of the expected size. A declared repetitive motif was confirmed in 92 loci by Sanger sequencing. The level of polymorphism was tested by fragment analysis. Statistical tests for observed and expected heterozygosities and deviations from Hardy-Weinberg equilibrium revealed 14 polymorphic microsatellite loci suitable for studies on the finer genetic structure of global populations of D. dendriticus.
摘要：
树枝状二头菌是鱼类传播的人畜共患病的病原体之一。多态微卫星标记最初是使用微卫星库筛选和下一代测序（NGS）为未来的遗传研究开发的。在NGS分析后选择的128个微卫星候选中，126产生预期大小的PCR产物。通过Sanger测序在92个基因座中确认了声明的重复基序。通过片段分析检测多态性水平。对观察到的和预期的杂合度以及与Hardy-Weinberg平衡的偏差进行的统计测试显示，有14个多态性微卫星基因座适合研究D.dendicus全球种群的更精细遗传结构。
